Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Plahsnig, D.
Effects of intraamniotically applied IGF-1 and -2 on protein expression in hypoplastic lungs of fetal rats with nitrofen-induced diaphragmatic hernia
Humanmedizin; [ Diplomarbeit ] Medical University of Graz; 2014. pp. 53
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- Autor*innen der Med Uni Graz:
- Betreuer*innen:
- Klaritsch Philipp
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- Abstract:
- Purpose: One in about every 2500 newborn babies suffers from the consequences of a Congenital Diaphragmatic Hernia (CDH), such as pulmonary hypoplasia and pulmonary hypertension. Insulin-like growth factors (IGFs) are hormones with the ability to stimulate and regulate growth and differentiation of miscellaneous tissues, among, the tissue of fetal lungs. This experimental study aimed to investigate the effects of intraamniotically administered human recombinant IGF-1 (rhIGF-1), respectively IGF-2 (rhIGF-2) on the lungs of rat fetuses with nitrofen induced CDH and resulting lung hypoplasia. Methods: Timed pregnant Sprague-Dawley rats were exposed either to 100 mg nitrofen dissolved in olive oil on E9.5 (treatment groups), or remained untreated (control group). On E19 and E20 0.2 µg of rhIGF-1 (IGF-1 group; n=10), rhIGF-2 (IGF-2 group; n=10) or saline (placebo group; n=5 for each treatment group) were injected into the amniotic fluid of the nitrofen-exposed fetuses. After caesarean delivery on E21.5 surviving fetuses were examined concerning the existence of a CDH, then euthanized and necropsy was performed. The CDH positive left lungs of the 10 fetuses in each of the two IGF-groups, the 5 fetuses in the associated placebo groups and the 5 fetuses in the control group were analyzed by means of immunohistochemistry to evaluate the expression of specific markers for lung development. Results: Protein expression of Ttf-1, SMA, Fgf-10, Ki67 and Pdpn was neither in the IGF-1 nor in the IGF-2 group significantly changed compared to placebos. The difference between the treatment groups and the untreated controls might largely be due to the effect of nitrofen and the surgical intervention. Conclusion: Summarizing the results of our study, intraamniotically applied IGF-1 and -2 showed no beneficial effect on the protein expression of specific markers of lung proliferation and differentiation.