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Reischies, F.
The Influence of different oxygen concentrations on GPR55 and MMP expression in the human placenta
Humanmedizin; [ Diplomarbeit ] Medical University of Graz; 2014. pp. 77 [OPEN ACCESS]
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Authors Med Uni Graz:: Reischies-Meikl Frederike Marie Josefine
Advisor:: Gauster Martin
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Abstract:: The G-protein coupled receptor (GPR)55 and its endogenous ligand lysophosphatidylinositol (LPI) has a modulating function on proliferation, differentiation and migration. These biological processes are important for the development of the human placenta. Matrix Metalloproteinases (MMPs) are enzymes capable of degrading the extracellular matrix, which is necessary for the implantation of the placenta into the uterine wall. The aim of this study was to investigate the influence of LPI and different oxygen concentrations on the expression of MMPs 2,7,9,19 and GPR55 in the human placenta. First, trimester placenta explants were incubated under different oxygen (2.5%, 8%, 12% and 21%) concentrations, in presence or absence of LPI for 48 hours. RNA was then isolated and quantitative RT-PCR performed to analyse the gene expression of MMPs 2, 7, 9, 19 and GPR55.