Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Liebmann, D.
Towards iron nanoparticle-based stem cell imaging in vivo
Humanmedizin; [ Diplomarbeit ] Medical University of Graz; 2014. pp. 68
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- Autor*innen der Med Uni Graz:
- Betreuer*innen:
- Reinisch Andreas
- Strunk Dirk
- Altmetrics:
- Abstract:
- Regenerative medicine is believed to offer a curative treatment for diseases by transplantation of cells with potential to regenerate or support damaged tissue. Therefore, necessary cell tracking tools have to be developed for answering therapeutically important issues like delivery route, cellular distribution pattern or the determination of engraftment. Magnetic resonance imaging (MRI) provides resolutions in the higher micrometer range, offers different contrast modifying methods even for cells, can be used not only in an experimental but also a preclinical setting and has only few limitations like longer acquisition times or a low specificity. This thesis examines ways of labeling mesenchymal stem and progenitor cells (MSPCs) and endothelial colony-forming progenitor cells (ECFCs) with contrast agents for MRI. Different formulations of ultrasmall superparamagnetic iron oxide (USPIO) particles, Molday Ion™, and superparamagnetic iron oxide particles (SPIOs), Resovist® were tested for their uptake. Cells were successfully labeled with Resovist® and subsequent assays showed no reduction of adipogenic, osteogenic and chrondogenic differentiation in MSPCs and network formation in ECFCs. The cellular detection limit of two different MRI sequences providing a high contrast-to-noise ratio in combination with a low acquisition time were studied with an in vivo mouse model. It was possible to find an MRI artifact created by cells labeled with high doses of SPIOs. An optimized MRI sequence with a postprocessing step, dedicated to this artifact, permitted the visualization of single cells thus making it an appropriate candidate for further cell tracking applications.