Medizinische Universität Graz - Research portal
Selected Publication:
Balci, C.
FEASABILITY OF THE OVINE FETAL MODEL FOR IN-SITU TISSUE ENGINEERING OF THE OESOPHAGUS
[ Diplomarbeit ] Medical University of Graz; 2013. pp. 39
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- Authors Med Uni Graz:
- Advisor:
- Saxena Amulya Kumar
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- Abstract:
- Background: Oesophageal replacement by surgical techniques such as gastric pull-up, intestinal or colon replacement is associated with morbidity as these techniques offer far from perfect solutions. The ovine model for oesophageal tissue engineering (TE) was selected by our group due to the advantages it offers in performing fetal interventions. The aim of this study was to investigate the feasibility of the ovine fetal model for in-situ TE of the oesophagus using the fetal omentum as a bioreactor. Methods: Fetus from Austrian Mountain Sheep were selected for interventions between the 120-130- day (Group-2, n=3) of gestation. The fetus was exteriorized and full thickness biopsies of 1-3 cm were obtained from the fetal oesophagus after which an end-end anastomosis was performed. The oesophageal biopsies obtained were processed to organoid units (OU) which were seeded on collagen scaffolds, tubularized and implanted into the fetal omentum. The fetus was returned and the pregnancy allowed to continue. Results: Outcomes of the surgical procedures can be summarized as follows: 5. Surgery is feasible and can be carried out safely in 120-130 day fetuses. 6. Cervical oesophageal biopsies of 1-3 cm were obtained in all 3 fetuses and the collagen construct could be successfully implanted into the abdomen. 7. Vascularization of the implanted contructs was achieved in all fetuses with the omental wrap. 8. There were no fetal mortalities in the fetal group operated between the 120- 130 day of gestation. Conclusion: Fetal interventions are feasible in ovine oesophageal tissue engineering. Biopsies of maximum 3 cm are feasible in 120-130 day fetuses.