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Mikorey, L.
Influence of diabetic conditions on the human placenta
[ Diplomarbeit ] Graz Medical University; 2012. pp. 116 [OPEN ACCESS]
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Autor*innen der Med Uni Graz:
Betreuer*innen:: Desoye Gernot; Gauster Martin
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Abstract:: Background: Diabetes in pregnancy causes pathologies in the human placenta and the fetus. Aim of this work was to investigate the effect of diabetic conditions on the expression of the MMPs and the enzymatic antioxidants in the human placenta. Material and Methods: Human placental explants of the first trimenon were cultivated under diabetic conditions, such as different glucose (16mM and 25mM) and oxygen concentrations (2.5%, 8%, 12% and 21%), and gene expression of MMP2, 7, 9 and 19 and the enzymatic antioxidants SOD3, NOX5 and GPX3 was subsequently analyzed via RT- PCR. The statistical evaluation was performed by SPSS for windows by using of Shapiro-Wilk- Test, Kolmogorov-Smirnof-Test (variant of Lilleforts), Mann-Lavene-Test, Mann- Whitney-U-Test, H-Test of Kruskal and Wallis, t-Test and ONEWAY ANOVA. Results: We could not find any significant changes in expression of the investigated genes under the different glucose and oxygen concentrations. Only certain trends in single genes may be assumed. Conclusion: The MMPs as well as the antioxidative enzymes play a role in the implantation of the placenta and may be influenced by diabetic conditions; nevertheless our experiments could not prove this hypothesis.