Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Grgic, R.
MICRO CONDUCTION MEASUREMENTS OF CARDIAC FIBROSIS IN ISOLATED RAT HEARTS
[ Diplomarbeit ] Medical University of Graz; 2012. pp. 88
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- Autor*innen der Med Uni Graz:
- Betreuer*innen:
- Andrä Michaela
- Wasler Andrae
- Altmetrics:
- Abstract:
- Cardiac diseases are the leading cause of mortality throughout the world. Fibrosis is an important structural substrate leading to various cardiovascular pathologies. The quantity and the quality of fibrosis strongly correlate with the appearance of rhythm disorders. Especially micro fibrosis could not be efficiently detected up to now, thus limiting the accuracy of catheter ablation. The present study aimed to detect micro fibrosis via cardiac near field measurement ¿for the first time in isolated perfused Langendorff rat hearts. Micro-conduction mapping methods as well as physiological measurements were performed on native (n=4) and on cyclosporine treated (n=6) rat hearts. The main objective was to show if a possible correlation between the morphology of electrograms and the amount of underlying fibrosis induced by cyclosporine treatment, could be estimated. In 50% of the cyclosporine treated rats, areas of minimal fibrosis were histopathologically confirmed. As expected this did not significantly affect the physiological cardiac performance. Nevertheless these minor structural changes could be detected through micro ¿conduction mapping using a new developed sensor. Significant differences were found. Following biophysical parameters: ¿¿(t)(p= 0,029), d¿¿(t)/dt (p= 0,002) were decreased, while the fractionation index was higher in the cyclosporine treated group. Although these findings need to be confirmed in future studies, they strongly imply the possibility to detect micro fibrosis and thereby offer a reasonable potential to be transferred into a clinical application.