Medizinische Universität Graz - Research portal
Selected Publication:
Aringer, I.
Expression of different adipocytokines in breast cancer, adjacent and distant fat tissue
[ Diplomarbeit ] Medical University of Graz; 2012. pp. 61
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- Authors Med Uni Graz:
- Advisor:
- Hrzenjak Andelko
- Moinfar Farid
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- Abstract:
- Introduction: Adipose tissue is an important endocrine organ producing bioactive peptides(proteins) known as adipocytokines (e.g.; leptin, interleukin-6, tumor necrosis factor-¿, adiponectin, resistin, serum amyloid A, etc.) with various metabolic roles. Deregulation of adipocytokines may lead to an elevated cancer risk. Overweight and obese postmenopausal women have a higher risk developing breast cancer. The question rises up how different adipocytokines are expressed in breast tumor tissue in comparison to adjacent and distant fat tissue. Materials & Methods: For this retrospective study frozen breast fat tissues (adjacent to breast tumor and ¿2 cm away from the tumor) and breast tumor tissues from 5 female patients were used and high-quality RNA was isolated. For quantitative mRNA expression analyses of different adipocytokines SYBR Green-based qRT-PCR was used and data were analysis by relative quantification using ¿¿Ct method. The target genes were normalized to the housekeeping gene ß2-microglobulin. Data for tumor and adjacent fat tissue samples were further normalized to the distant fat, defined as healthy tissue. Statistics and graphics were edited in Excel. Results: We observed that in tumor tissue samples following adipocytokines were downregulated: leptin, adiponectin, IL-6 and serum-amyloid. Whereas in tumour tissue IL-6 was downregulated, it was up-regulated in all adjacent fat tissue samples. Serpin expression was up-regulated in tumour tissues in four patients. Discussion & Conclusion: In most cases we observed a down regulation of adipocytokines in tumor tissue in comparison to the distant fat tissue. Data for adipocytokines expressions in adjacent fat tissue samples were inconsistent, showing the large biological heterogeneity between different patients. Because of the limited number of samples it is difficult to make a clear statement. However, our data indicate that cancer cells might effect adipocytokin expression not only in the tumor but also in the adjacent fat tissue. In context with different patho/physiological roles of adipocytokines this might influence tumor development and growth.