Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Klimbacher, G.
Comparative studies on esophageal glands in animal models for regenerative medicine applications
[ Diplomarbeit ] Medical University of Graz; 2012. pp. 44
[OPEN ACCESS]
FullText
- Autor*innen der Med Uni Graz:
- Betreuer*innen:
- Saxena Amulya Kumar
- Altmetrics:
- Abstract:
- Background: Esophagus tissue engineering holds promises in the future to overcome the limitations of the presently employed gastrointestinal surgical replacement procedures. Although major attention is being focused towards the engineering of the esophagus with esophageal epithelial cell, smooth muscle cells and nerve tissue; scant attention is being paid to esophageal glands (EG). This study aimed to investigate different animal models with regard to EG and to identify tissues appropriate for regenerative medicine applications. Methods: Esophagus was obtained from ovine, avian, bovine, murine and porcine sources and investigated with regards to the presence of EG. Histological specimens were stained using Hematoxylin-Eosin (HE) to locate EG in the upper, middle and lower portions of each esophagus. If EG were identified, the tissue was further investigated using Periodic Acid Schiff (PAS) and Alcian Blue (AB) to determine the presence of neutral or acidic glands. AB at 3 pH levels (0.2, 1.0 and 2.5), was used to detect the presence of sulphated mucous. The Olympus Cell^F® was then used to measure the size, the perimeter, the shortest distance to lumen and the concentration of acini in EG. These results were compared to data on human EG and utilized to determine scaffold characteristics for EG in esophagus tissue engineering. Results: The esophagus taken from murine, bovine and ovine sources were devoid of EG. In avian esophagus, acidic and sulphated mucous producing EG were identified throughout the esophagus with larger EG located cranially. On the other hand, in porcine esophagus predominantly neutral and sulphated mucous producing EG with isolated pockets of acidic and sulphated mucous were identified, in the cranial and middle areas of the esophagus. Distance of EG to lumen ranged from 127-340 ¿m (avian) to 916-983 ¿m (porcine). EG consisted of 35% (avian) to 45% (porcine) area of the submucosa. EG had an area of 125000 ¿m2 (avian) and 580000 ¿m2 (porcine). Conclusion: The results of these investigations demonstrate that porcine esophagus is optimal for EG investigation in terms of scaffold development for tissue engineering. The neutral sulphated mucous in porcine esophagus correlates well with data available on human EG.