Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Bubik, S.
Dynamic bioreactor tests in regenerative medicine: Investigations with biodegradable collagen scaffolds
[ Diplomarbeit ] Medical University of Graz; 2012. pp. 66
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- Autor*innen der Med Uni Graz:
- Betreuer*innen:
- Saxena Amulya Kumar
- Altmetrics:
- Abstract:
- Background: Research in tissue engineering holds promises to generate tissue or whole organ substitutes for the treatment of congenital anomalies and acquired defects due to disease and trauma. Techniques are being developed to grow new tissue in-vitro and in-vivo. There are 2 in-vitro systems that are applied for tissue engineering: static and dynamic. The aim of this study was to perform preliminary investigation with regards to the mechanical stability of collagen scaffolds. Two different scaffolds (dense and porous) were employed in a dynamic bioreactor system using ovine esophageal smooth muscle cells (OESMC). Methods: Sterilized bovine pericardium (dense) and a fabricated porcine collagen sponge (porous) were used as organic scaffolds for investigations. The scaffolds were sutured to formed tubes, seeded with OESMC and then set in a special tissue chamber that was attached to a dynamic bioreactor pump system. The constructs were exposed to dynamic bioreactor conditions after which the scaffolds were investigated under various aspects: (a) Ability to tubularize scaffolds on media feeding ends of the bioreactor, (b) Success in maintaining natural and fabricated collagen scaffolds in a bioreactor, (c) Cell attachment under minimal flow conditions offered by the bioreactor and (d) Structural changes evident in natural and fabricated collagen scaffolds. Results: Both bovine pericardium and the porcine collagen sponge were successfully tubularized for bioreactor investigations and successfully maintained during the 7 day period. Cell attachment was not possible since the minimal flow settings offered by the pump system was extremely high which led to cell detachment. The structure of both scaffolds showed little deformations, thus confirmed their suitability for use in such systems. Conclusion: Dynamic bioreactors are important to generate tissue under improved culture conditions than those offered by static cultures. There are many factors that influence the success of tissue formation in dynamic bioreactors which need to be considered when using such systems in tissue engineering.