Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Betge, J.
Gastric cancer and concomitant renal cancer:
A systematic clinicopathological,immunohistochemical and molecular analysis
[ Diplomarbeit ] Medical University of Graz; 2012. pp. 83
[OPEN ACCESS]
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- Autor*innen der Med Uni Graz:
- Betreuer*innen:
- Höfler Gerald
- Langner Cord
- Altmetrics:
- Abstract:
- Objective: The frequency of gastric cancer in patients with renal cell carcinoma (RCC) is remarkably high in our area suggesting a common molecular basis. Our study aimed to characterize tumors and to analyze possible underlying molecular features in twelve patients with gastric cancer and concomitant RCC. Methods: A histological and immunohistochemical analysis was performed, which included p53 protein expression, proliferative activity (MIB-1), mismatch repair status (hMLH1, hMSH2, hMSH6, PMS2) and E-cadherin expression for gastric cancers, which were additionally analyzed for Epstein-Barr-Encoded-RNA (EBER) by in-situ hybridization. Microsatellite instability was analyzed with a PCR multiplex system and capillary electrophoresis. KRAS mutations in codons 12 and 13 were tested by pyrosequencing. Results: All patients had clear cell RCCs, ten of which were well differentiated and diagnosed in an early stage, while the patients¿ gastric cancers were generally poorly or undifferentiated and diagnosed in an advanced stage. Gastric cancers showed reduced E-cadherin staining in ten out of twelve cases. Two gastric cancers demonstrated loss of hMLH1 and PMS2, which was confirmed by molecular analysis showing a high degree of microsatellite instability. All RCCs were microsatellite stable. KRAS mutation was detected in one of the two instable gastric cancers, while none of the RCCs had KRAS mutations. Another gastric cancer was positive for EBV. Conclusions: In conclusion, a coherent cause for gastric cancer and concomitant RCC, such as Lynch syndrome, a prominent role of KRAS mutation or EBV infection, was not found in our series. Other factors leading to a higher susceptibility for cancer will have to be explored to explain why people with RCC have a higher risk to develop gastric cancer in our area.