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Pirker, G.
Assessment of the intestinal microcirculatory disturbances during sepsis and obesity
[ Diplomarbeit ] Medical University of Graz; 2011. pp. 42 [OPEN ACCESS]
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Authors Med Uni Graz:
Advisor:: Ruttenstock Elke Maria; Singer Georg
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Abstract:: In the last years the world is confronted with an ever increasing incidence of obesity. Obesity is associated with a decreased life expectancy, the development of several other diseases and high costs of care. Sepsis is a common disease that is associated with high mortality and high costs of care and is the leading cause of death in non-coronary intensive care units as well as the 10th leading cause of death in the United States. Alterations of the microcirculation have been shown to play a significant role in the development of sepsis in obesity models. The aim of the present diploma thesis was to describe the intestinal microcirculatory disturbances during sepsis in two strains of obese mice. Male C57BL/6J@Rj-ob (ob/ob) and male C57/KsJ@Rj-db (db/db) mice were used and compared to male C57BL/6J Rj (WT) mice. Sepsis was induced by cecal ligation and puncture. Weight, circulating blood cells and intra-peritoneal bacteria, wet-dry ratio of the lung and leukocyte rolling and adhesion of the small intestine was observed 6 hours following CLP and values were compared between septic obese mice and a group of WT mice following CLP and a sham operation respectively. Survival following surgery was observed for 168 hours. There was no significant difference between the four groups in the amount of circulating blood cells and the wet-dry ratio of the lung. The WT CLP group showed significantly more intra-peritoneal bacteria compared to the other groups. The amount of adherent leukocytes was significantly higher in groups experiencing CLP surgery when compared to the sham group. Leukocyte rolling did not significantly differ among the groups though there was a trend towards increased rolling in the CLP groups. A markedly decreased survival could be seen in both obese groups following induction of sepsis.