Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Leitner, E.
Molecular detection of bacteria and fungi in blood samples: comparison of two molecular assays with conventional blood culture
[ Dissertation ] Medical University of Graz; 2011. pp.79.
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- Autor*innen der Med Uni Graz:
- Leitner-Meyer Eva
- Betreuer*innen:
- Kessler Harald
- Krause Robert
- Altmetrics:
- Abstract:
- Background: Rapid and accurate diagnostics to identify microorganisms responsible for blood stream infections are essential for accurate antimicrobial therapy. Blood culture is the current gold standard for diagnosis of microorganisms in blood. New molecular tests have been developed which may accelerate diagnosis. Objectives: The aim of this study was to compare molecular assays, the LightCycler® SeptiFast Test MGRADE and SepsiTestTM Blood, with conventional blood culture for detection of microorganisms producing bloodstream infections. Study design: Seventy-five specimens were collected from patients with or without sepsis and tested with the LightCycler® SeptiFast Test MGRADE, the SepsiTestTM Blood, and conventional blood culture. Results obtained were compared. Results: All tests gave negative results for 49 of 75 (65%) specimens with 15 of 26 (57%) in the sepsis group and 34 of 49 (69%) in the non-sepsis group. Positive results were obtained for 2 of 75 (3%) specimens with all tests in the non-sepsis group. For 24 of 75 (32%) specimens, discrepant results were obtained. When all tests were compared, an overall agreement of 68% was found. Conclusions: The molecular assays provided a more rapid detection and identification of microorganisms responsible for bloodstream infections when compared to blood culture. The LightCycler® SeptiFast Test MGRADE requires fewer hands on time and offers better-defined identification results, whereas the SepsiTestTM Blood is capable of detecting a larger number of microorganisms. However, due to the need of susceptibility testing for appropriate antimicrobial treatment, molecular tests do not have the potential to replace the current gold standard blood culture.