Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Neun, U.
Untersuchung der Wachstumsfuge nach transepiphysärer Bohrung-Etablierung der Kollagenfärbung I und II
[ Diplomarbeit/Master Thesis ] Medical University of Graz; 2010. pp. 83
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- Autor*innen der Med Uni Graz:
- Betreuer*innen:
- Amerstorfer Eva
- Weinberg Annelie-Martina
- Altmetrics:
- Abstract:
- Introduction: Fractures involving the physeal growth plate is a daily clinic problem. Damage to this fargile structure might cause growth arrest wich lead to limb deformities. Although there¿s intensiv research at this topic, the background of the formation of the bone bridge is still a mystery. Methodes and Material: 42 Sprague-Dawley rats at the age of 6 weeks and a mass of 100 up to 120g were splitted in 7 groups by random. Five rats were operated and one was used as control. After drilling an axial hole through the proximal epiphyses of the left tibia, the rats were euthanized on day 1, 3, 7,14, 28, 42, 82. Bones were extracted carefully. After the decalcification slides were made with a cryomicrotom. Histological staining was made with collagen I (Sigma®) and collagen II (Iowa®) antibodies according to the protocol. All sections were analyzed under the microscop. Results: On the first postoperativ day there is hematoma and bone fragments caused by drilling. On day 3 you can find a lot of inflammatory cells and an organizing hematoma. The first signs for new bone tissue appear on day 7. You can also find mesenchymal cells. Joining the fracture zone there are a few chondrocyte like cell with positiv collagen II immunostaining. The cells can be seen until day 42. On day 14 bone trabeculae cross the epiphyseal growth plate. A mature bone bridge had been found on day 28 which fills up the whole lesion on day 82. Discussion: The formation of a bone bridge was documented by this research. The first signs of a new bone tissue appear on day 7 and a mature bone bridge can be found on day 28. The origin of this bone tissue is still not clear. The condrocyte like cells which were found along the lesion might be a hint that the bone bridge is formed by enchondral ossification, but furhter investigation is necessary to proof it.