Medizinische Universität Graz - Research portal
Selected Publication:
Sulejmanovic, A.
Comparison of collagen Scaffolds after in-vivo ovine Implantation: Impact on hollow organ Tissue Engineering
[ Diplomarbeit ] Medical University of Graz; 2010. pp. 57
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- Authors Med Uni Graz:
- Advisor:
- Saxena Amulya Kumar
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- Abstract:
- Background: Management of congenital birth defects like short bowel syndrome, long-gap esophageal atresia or dysplasia of the urinary tract poses challenges. Surgical techniques to manage them are frequently associated with complications and high morbidity. In the above aforementioned defects tubular Scaffolds represent a central role and could serve as a tissue replacement. The aim of this study was to investigate collagen Scaffolds of various densities with regards to tissue integration after in-vivo implantation. Methods: Scaffolds investigated were: (a) Porous Collagen Scaffold (control), (b) Polymer-Collagen Scaffold (hybrid) and a (c) compressed dual layer Scaffold (dual layer). The dual layer had a non porous luminal layer and the hybrid Scaffold showed full incorporation of the PCL knitting. Ultimate tensile strength and suture retention for hybrid was significantly higher than for other constructs. The Scaffolds were sutured into tubes using stents and implanted into the ovine omentum for a period of 2 and 4 weeks. The tubes were explanted and investigated with regards to their integration characteristics. Studies were performed after ethical committee permission. Results: The hybrid Scaffold demonstrated minimal cellular infiltration and proliferation. The Dual Layer Scaffold was found to be the most suitable collagen Scaffold for hollow organ tissue engineering when investigated under in-vivo conditions in the ovine model. Also the control Scaffold demonstrated good results with regards to integration. It is noteworthy that the three Scaffolds were well integrated into the ovine omentum with minimal inflammatory responses. Conclusion: The distinct properties of various collagen Scaffolds can be applied according to their demand in tissue engineering. The dual layer has been found to be best suited for gastrointestinal tissue engineering. Properties of the three Scaffolds make them suitable for pre-seeding.