Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Tilz, H.
Gene Exression Profiling in Melanoma Cell Lines
[ Dissertation ] Medical University of Graz; 2004. pp.
- Autor*innen der Med Uni Graz:
- Betreuer*innen:
- Reibnegger Gilbert
- Schaider Helmut
- Altmetrics:
- Abstract:
- Melanomas develop from abnormally growing melanocytes. Although prevention is improving gradually and melanomas are being diagnosed earlier, mortality in patients with advanced melanoma is still high. Apart form environmental factors, inherited factors are suggested to play causative role in some melanomas. Genetic investigations have revealed stereotypical mutations that are associated with melanoma. Genes, such as CDKN2A or Bcl2 are implicated in melanomagenesis, but we are still at the beginning to gain insight into the pathogenesis on the molecular level. With the use of gene expression profiling, the molecular expression of genes can be visualized more easily. The microarray method enables us to produce the expression profiles of thousands of genes. This technique was used to examine the gene expression in tow melanoma cell lines of different tumorigenic potential. A primary, non-tumorigenic melanoma cell line and a metastatic melanoma cell line were probed for gene expression, using 60-mers oligonucleotide microarrays. The array represents 17 575 genes and enabled us to identify a set of genes with different expression profiles in both cell lines. The analysis of the genes proved that 488 genes were up-regulated in the primary tumor. We also discovered 1784 down-regulated genes in the primary tumor versus the metastatic tumor. Of the 488 genes, which were up-regulated, we found particular genes which are specific interest such as MAGEA10, IL1-Beta, CTGF and FFPI-2. Of the 1784 genes, which were down-regulated, we identified SILV, MITF, MIA, S100beta, p19ARF and tyrosinase as the genes suggested to involved in melanoma development. These results show that (I) the gene expression profile in the primary melanoma cells differ widely in comparison to the expression in the metastatic cells, - (II) the down regulated genes are indicators of dedifferentiation in the sequence of tumor development and (III) new genes, which may be important for melanoma progression, such as Connexin 26, annexin I, and TRIMM22 have been identified.