Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Neuwersch, S.
Polymorphisms in IL-10 and TNF-Alfa and the risk of spontaneous preterm birth
[ Dissertation ] Medical University of Graz; 2007. pp.
- Autor*innen der Med Uni Graz:
- Neuwersch-Sommeregger Stefan Matthias
- Betreuer*innen:
- Pertl Barbara
- Schöll Wolfgang
- Altmetrics:
- Abstract:
- BACKGROUND Infection and inflammatory cytokines play a central role in the pathogenesis of preterm birth. Elevated levels of the pro-inflammatory cytokine TNF- and decreased levels of the anti-inflammatory cytokine IL-10 have been associated with preterm birth. The TNF- TNFA2 allelic variant, the IL-10 (-1082) (G/A) polymorphism, and the significantly and thus, influences the inflammatory response. Different inconsistent studies encouraged us to investigate the association between the TNF- and the IL-10 polymorphisms and preterm delivery. We hypothesized neonatal polymorphisms also influence the risk preterm birth. PATIENTS AND METHODS 75 women who delivered earlier than 35 weeks of gestation (between 2002 and 2007) and their children and 222 women with term birth after 37 weeks of gestation (between 2006 and 2007) and their children participated in this case-control study. Eligibility for the preterm birth group required spontaneous preterm labor and delivery before 35 completed weeks of gestation. Also women with PROM, AIS, cervical insufficiency and preterm delivery were included. The control group enrolled women with term birth after 37 weeks of gestation and no preterm deliveries in their case history. Exclusion criterions were gestosis, non spontaneous labor, uterine malformations, previous uterine surgery, placental abruption, placental insufficiency, placenta previa, umbilical cord prolaps, UIGR, abnormalities of the child, chronic infection diseases like hepatitis B/V or HIV infection and in vitro fertilization treatment. Analysis of IL-10 (-1082), IL-10 (-819) and TNF- (-308) polymorphisms was performed in cases and controls from venous blood samples from every mother as well as two buccal swabs and umbilical cord blood from every newborn baby after parental informed consent. The study was approved by the local ethic committee. All probes were given a code number and analysis was done anonymously at the Center for Medical Research Graz (ZMF). Genotyping was performed using PCR technique and gel electrophoresis. RESULTS In this case-control study no significant differences were found for maternal polymorphisms of IL-10 (-1082), IL-10 (-819) and TNF- (-308) between cases and controls. The same results were found for neonatal polymorphisms of IL-10 (-819) and TNF- (-308). Neonatal polymorphisms of IL-10 (-1082) for (A/A) mutations were found at a higher rate in cases compared to controls, but the difference did not reach statistical significance (p=0.056).