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SHR Neuro Cancer Cardio Lipid Metab Microb
Donnerer, J; Liebmann, I.
Dorsal root ganglion neurons respond with prolonged extracellular signal-regulated protein kinase phosphorylation following noxious heat and cold stimulation.
Neurosci Lett. 2010; 472(2): 109-113.
Doi: 10.1016/j.neulet.2010.01.064
Web of Science
PubMed
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FullText_MUG
- Leading authors Med Uni Graz
- Donnerer Josef
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- Abstract:
- In the present study the time course of extracellular signal-regulated protein kinase phosphorylation (pERK1/2 appearance) in lumbar sensory dorsal root ganglia (DRG) was determined following a 5-min noxious heat or a noxious cold stimulus to the hind paw of the rat. The thermal stimuli were chosen to activate transient receptor potential (TRP) channels, but not to induce tissue damage. A quantitative analysis of phospho-ERK1/2 was performed by protein extraction and Western blot analysis. Western blot analysis showed that following the heat stimulus, phosphorylation of ERK1/2 increased 2-3-fold between 10 and 30min in the DRG on the ipsilateral side. High levels were maintained from 30min up to 16h. Following the cold stimulus to the paw, pERK1/2 immediately increased 2-fold within 2min in the DRG on the ipsilateral side, it declined within 2h and reached a second peak at 4h. In the DRGs on the contralateral side of the paw's heat or cold immersion the pERK1/2 remained low at all time points investigated. Fluorescence immunohistochemistry of the DRG following the thermal stimuli revealed an increased cytoplasmic staining for pERK1/2 in neurons. The present results show that following a 5-min nociceptive thermal stimulus sensory neurons respond with a characteristic long-lasting phosphorylation of ERK1/2.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Cold Temperature -
- Ganglia, Spinal - cytology
- Hot Temperature -
- Lumbosacral Region -
- Male -
- Mitogen-Activated Protein Kinase 1 - metabolism
- Mitogen-Activated Protein Kinase 3 - metabolism
- Phosphorylation -
- Rats -
- Rats, Sprague-Dawley -
- Sensory Receptor Cells - metabolism
- Find related publications in this database (Keywords)
- Intracellular signalling
- ERK1/2 phosphorylation
- TRP channels
- Western blotting
- Immunohistochemistry