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Selected Publication:
BECKER, JC; DUMMER, R; VONWUSSOW, P; BURG, G; SCHMIDT, RE.
NON-MHC-RESTRICTED T-CELL INTERACTION WITH B-CELLS - ROLE OF THE T-CELL RECEPTOR
SCAND J IMMUNOL. 1992; 36(2): 177-181.
Doi: 10.1111/j.1365-3083.1992.tb03089.x
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- Leading authors Med Uni Graz
- Becker Jürgen Christian
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- Abstract:
- Non-specific antibody production usually accompanies the T-cell-regulated B-cell response. In this paper the mechanisms involved in non-MHC-restricted T-B-cell interaction were studied. As previously shown for NK cells, activated B cells induce IFN-gamma and TNF-alpha production in non-MHC-restricted cytotoxic T lymphocytes (NrCTL). Using an in vitro model system, we demonstrate that direct cell-cell interactions are required to induce these cytokines in NrCTL. Receptor ligand systems involved are leucocyte function antigen-1/intercellular adhesion molecule-1 (LFA-1/ICAM-1) (CD11a,CD18/CD54), T11/LFA-3(CD2/CD58), and the clonotypic T-cell receptor (TCR) structure NKTa of JT9/JT10 with its non-MHC-related target antigen TNKtar (4F2). Cytokine production can be induced by activating monoclonal antibodies against CD2R. Antibodies against the clonotypic TCR (NKTa) or CD3 had no cytokine-inducing effect on NrCTL cultured alone, but were able to retrieve the effect of blocking the target antigen on cocultured B cells. We could further demonstrate that the inhibition of the TCR/target antigen interaction could be overcome by close cell-cell contact culture conditions. From these findings it is concluded that the role of the TCR in non-MHC-restricted cell-cell interaction is to facilitate LFA-1/ICAM-1-mediated effector target adhesion in a specific way rather than to mediate direct activating signals upon lymphokine production or cytoxicity.