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BECKER, JC; SCHWINN, A; DUMMER, R; BURG, G; BROCKER, EB.
TUMOR-INFILTRATING LYMPHOCYTES IN PRIMARY MELANOMA - FUNCTIONAL CONSEQUENCES OF DIFFERENTIAL IL-2 RECEPTOR EXPRESSION
CLIN EXP IMMUNOL. 1993; 91(1): 121-125. Doi: 10.1111/j.1365-2249.1993.tb03365.x
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Leading authors Med Uni Graz: Becker Jürgen Christian
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Abstract:: Tumour-infiltrating lymphocytes (TIL) have been isolated from early primary melanoma (Clark level III) and expanded in vitro using culture conditions with low concentrations of IL-2 (50 U/ml). Immediately after isolation TIL consisted of mainly CD3+ T cells, and the portion of CD56+ natural killer (NK) cells was below 20%. Fresh TIL cultures could be distinguished by CD25 expression since some contained up to 33%, others less than 5% CD25+ cells. These showed differences in subsequent development during in vitro expansion. CD25-expressing cultures remained stable in their phenotype, whereas the second TIL type showed major changes: CD3 (ca 70-30%) expression decrease, CD25 (ca 5-35%) and CD56 (ca 15-55%) expression increase. The TIL type, which remained dominated by CD3+ T cells, killed autologous tumour cells efficiently (Cr-51-release greater than 30% at a E/T ratio of 20:1), which could be blocked by MoAbs against MHC class I molecules. In contrast, the other TIL type exhibited weak cytotoxicity (less than 17% Cr-51-release at an E/T ratio of 20:1) against the autologous tumour. Therefore, the expression of CD25 on freshly isolated TIL is a good marker for tumour specificity of in vitro expanded TIL.
Find related publications in this database (Keywords): TUMOR-INFILTRATING LYMPHOCYTES; PRIMARY MELANOMA; IL-2 RECEPTOR