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DUMMER, R; BECKER, JC; EILLES, C; SCHAFER, E; BORNER, W; BURG, G.
T-CELLS MIGRATE TO TUMOR SITES AFTER EXTRACORPOREAL INTERLEUKIN-2 STIMULATION AND REINFUSION IN A PATIENT WITH METASTATIC MELANOMA
BRIT J DERMATOL. 1993; 128(4): 399-403. Doi: 10.1111/j.1365-2133.1993.tb00198.x
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Co-authors Med Uni Graz: Becker Jürgen Christian
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Abstract:: Peripheral blood mononuclear cells (PBMC) were taken by leukapheresis from a patient with melanoma skin metastases and stimulated in vitro using 1000 IU recombinant interleukin 2 (IL-2)/ml to generate lymphokine-activated killer cells (LAK cells). Two-colour immunofluorescence analysis demonstrated an IL-2-induced up-regulation of CD25 on natural killer cells (CD56+) as well as on T lymphocytes (CD3-). After radiolabelling with indium-111, the cells were reinfused. Gamma-camera imaging revealed an enrichment at the tumour sites. Immunostaining of tumour tissue taken before and after scintigraphy demonstrated CD25- T lymphocytes (CD2+, CD3+). but no natural killer cells (CD16+, CD56+) infiltrating the metastases. LAK cell enrichment at melanoma metastases in vivo did not involve natural killer cells, but was characterized by increased numbers of activated T lymphocytes in this patient.