Medizinische Universität Graz - Research portal
Selected Publication:
BECKER, JC; KOLANUS, W; LONNEMANN, C; SCHMIDT, RE.
Human natural killer clones enhance in vitro antibody production by tumour necrosis factor alpha and gamma interferon.
SCAND J IMMUNOL. 1990; 32(2): 153-162.
Doi: 10.1111/j.1365-3083.1990.tb02905.x
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- Leading authors Med Uni Graz
- Becker Jürgen Christian
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- Abstract:
- To determine whether NK cells are involved in the regulation of the antibody response, we studied the effects of human NK clones on B-cell growth and differentiation and the mechanisms involved. We demonstrate that various human NK clones enhance the immunoglobulin production of SAC/rIL-2-activated B cells, e.g. IgG and IgM by up to 230% and anti-tetanus toxoid antibodies by up to 430%. Cell-cell interactions via cell-surface structures, e.g. the CD11a/CD18 molecule, were found to be critical. Subsequently the NK-mediated B-cell regulation involves cytokines, since cell-free supernatants obtained by 48-h cultures of NK clones exerted BCGF and BCDF activity. Neutralization studies and direct determination characterized these cytokines as IFN-gamma and TNF-alpha. The cytokine production of NK clones could be triggered by activated B cells only. Northern blot analysis demonstrated that activated B cells in co-culture with NK clones were able to induce accumulation of mRNA transcripts for IFN-gamma and TNF-alpha in NK cells.
- Find related publications in this database (using NLM MeSH Indexing)
- Antibodies, Monoclonal -
- Antibody Formation -
- Antigens, CD11 -
- Antigens, CD18 -
- Antigens, Differentiation - physiology
- B-Lymphocytes - cytology B-Lymphocytes - immunology
- Blotting, Northern -
- Cell Communication -
- Clone Cells -
- Humans -
- Interferon-gamma, Recombinant - biosynthesis Interferon-gamma, Recombinant - genetics
- Killer Cells, Natural - immunology
- Lymphokines - immunology
- RNA, Messenger - biosynthesis
- Receptors, Leukocyte-Adhesion - physiology
- Transcription, Genetic -
- Tumor Necrosis Factor-alpha - biosynthesis Tumor Necrosis Factor-alpha - genetics