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Selected Publication:
DUMMER, R; POSSECKERT, G; NESTLE, F; WITZGALL, R; BURGER, M; BECKER, JC; SCHAFER, E; WIEDE, J; SEBALD, W; BURG, G.
Soluble interleukin-2 receptors inhibit interleukin 2-dependent proliferation and cytotoxicity: explanation for diminished natural killer cell activity in cutaneous T-cell lymphomas in vivo?
J INVEST DERMATOL. 1992; 98(1): 50-54.
Doi: 10.1111/1523-1747.ep12494223
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- Co-authors Med Uni Graz
- Becker Jürgen Christian
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- Abstract:
- In patients with cutaneous T-cell lymphomas (CTCL), soluble interleukin-2 receptor serum levels (sIL-2R) were determined by ELISA technique, and natural killer cell (NK) activity, by a 4-h chromium-51 release assay. Decrease of NK activity correlated with the augmentation of serum sIL-2R. After a 4-d stimulation with interleukin 2 CTCL patients' peripheral mononuclear cells (PMC) showed an increase of cytotoxic activity similar to that in healthy donors' PMC. Normal donors' PMC demonstrated a diminished IL-2-induced cytotoxic activity in 25% CTCL serum (sIL-2R of 3000, 7330, and 10700 U/ml, respectively) compared to control serum (sIL-2R of 400, 340, and 420 U/ml, respectively). IL-2-dependent proliferation of 2-d phytohemagglutinin (PHA) blasts was lower in CTCL serum than in control serum. sIL-2R was enriched from one CTCL patient's serum by IL-2 affinity chromatography. Transfection of the Tac gene into NIH/3T3 fibroblasts resulted in the production of a recombinant sIL-2R. The presence of enriched native or recombinant sIL-2R inhibited interleukin-2-dependent generation of cytotoxic activity and PHA blast proliferation. We suggest that elevated sIL-2R levels account for diminished NK activity by neutralizing interleukin 2 in CTCL patients.
- Find related publications in this database (using NLM MeSH Indexing)
- Cytotoxicity, Immunologic -
- Humans -
- Interleukin-2 - antagonists and inhibitors
- Killer Cells, Natural - immunology
- Lymphocyte Activation -
- Lymphoma, T-Cell, Cutaneous - immunology
- Receptors, Interleukin-2 - analysis Receptors, Interleukin-2 - isolation and purification Receptors, Interleukin-2 - physiology
- Skin Neoplasms - immunology