Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
BECKER, JC; DUMMER, R; HARTMANN, AA; BURG, G; SCHMIDT, RE.
Shedding of ICAM-1 from human melanoma cell lines induced by IFN-gamma and tumor necrosis factor-alpha. Functional consequences on cell-mediated cytotoxicity.
J Immunol. 1991; 147(12):4398-4401
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- Führende Autor*innen der Med Uni Graz
- Becker Jürgen Christian
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- Abstract:
- ICAM-1-mediated cell-cell adhesion is essential for various immunologic functions, including non-MHC-restricted cytotoxicity. The present study was designed to establish whether shedding of ICAM-1 from melanoma cells occurred and to characterize the effects of soluble ICAM-1 on some cell adhesion-dependent functions. The shed soluble ICAM-1 molecule was detected and quantified by a specific ELISA. Shedding of ICAM-1 could be induced by IFN-gamma and TNF-alpha alone, or more effectively, by a combination of the two cytokines together. The use of purified soluble ICAM-1 enabled us to test for the functional significance of the ICAM-1 shedding from tumor cells. Conjugate formation between the cloned NK cell line CNK6 and the erythromyeloid cell line K562, as well as between lymphokine-activated killer cells and the melanoma cell line M26, could be inhibited by purified soluble ICAM-1 and cell-free supernatants from melanoma cell cultures containing shed ICAM-1. Furthermore, the non-MHC-restricted cytotoxicity mediated by NK and lymphokine-activated killer cells could be abrogated either by purified soluble ICAM-1 or by melanoma cell culture supernatants containing shed ICAM-1. Thus, shedding of ICAM-1 may be one of the mechanisms by which neoplastic cells escape immunosurveillance.
- Find related publications in this database (using NLM MeSH Indexing)
- Cell Adhesion - drug effects
- Cell Adhesion Molecules - analysis Cell Adhesion Molecules - physiology
- Cytotoxicity, Immunologic - drug effects
- Humans -
- Intercellular Adhesion Molecule-1 -
- Interferon-gamma - pharmacology
- Melanoma - immunology
- Tumor Cells, Cultured -
- Tumor Necrosis Factor-alpha - pharmacology