Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Becker, JC; Houben, R; Vetter, CS; Brocker, EB.
2-D differential membrane proteome analysis of scarce protein samples.
Proteomics. 2006; 6(16):4506-4513
Doi: 10.1002/pmic.200600169
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- Leading authors Med Uni Graz
- Becker Jürgen Christian
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- Abstract:
- Proteome studies with small sample amounts are difficult to perform, especially when membrane proteins are the focus of interest. In our study a new method for the analysis of scarce membrane protein samples combining large gel 2-D-CTAB/SDS-PAGE with fluorescence dye saturation labelling (satDIGE) was developed, allowing a highly sensitive differential analysis of different cell states. After Triton X-114 phase partitioning, enriched membrane protein samples of T cells were labelled at cysteine residues using fluorescence dyes and separated by large gel 2D-CTAB/SDS-PAGE. For a differential analysis 3 mug protein was found to be sufficient to detect proteins in a widespread well-separated diagonal spot pattern.
- Find related publications in this database (using NLM MeSH Indexing)
- Amino Acid Sequence -
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- Fluorescent Dyes - chemistry
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- Membrane Proteins - analysis Membrane Proteins - isolation and purification
- Mice -
- Molecular Sequence Data -
- Proteome -
- Spectrometry, Mass, Electrospray Ionization -