Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Becker, JC; Houben, R; Vetter, CS; Brocker, EB.
2-D differential membrane proteome analysis of scarce protein samples.
Proteomics. 2006; 6(16):4506-4513
Doi: 10.1002/pmic.200600169
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Becker Jürgen Christian
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Proteome studies with small sample amounts are difficult to perform, especially when membrane proteins are the focus of interest. In our study a new method for the analysis of scarce membrane protein samples combining large gel 2-D-CTAB/SDS-PAGE with fluorescence dye saturation labelling (satDIGE) was developed, allowing a highly sensitive differential analysis of different cell states. After Triton X-114 phase partitioning, enriched membrane protein samples of T cells were labelled at cysteine residues using fluorescence dyes and separated by large gel 2D-CTAB/SDS-PAGE. For a differential analysis 3 mug protein was found to be sufficient to detect proteins in a widespread well-separated diagonal spot pattern.
- Find related publications in this database (using NLM MeSH Indexing)
- Amino Acid Sequence -
- Animals -
- Cells, Cultured -
- Electrophoresis, Gel, Two-Dimensional -
- Fluorescent Dyes - chemistry
- Humans -
- Membrane Proteins - analysis Membrane Proteins - isolation and purification
- Mice -
- Molecular Sequence Data -
- Proteome -
- Spectrometry, Mass, Electrospray Ionization -