Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Hofmeister-Mueller, V; Vetter-Kauczok, CS; Ullrich, R; Meder, K; Lukanidin, E; Broecker, EB; Straten, PT; Andersen, MH; Schrama, D; Becker, JC.
Immunogenicity of HLA-A1-restricted peptides derived from S100A4 (metastasin 1) in melanoma patients.
Cancer Immunol Immunother. 2009; 58(8):1265-1273
Doi: 10.1007/s00262-008-0640-0
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Becker Jürgen Christian
- Schrama David
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- S100A4 (metastasin 1) belongs to the S100 family of Ca(2+) binding proteins. While not present in most differentiated adult tissues, S100A4 is upregulated in the micromilieu of tumors. It is primarily expressed by tumor-associated macrophages, fibroblasts, and tumor endothelial cells. Due to its strong induction in tumors S100A4 is a promising target for cancer immunotherapy. By reverse immunology, using epitope prediction programs, we identified 3 HLA-A1-restricted peptide epitopes (S100A4 A1-1, A1-2, and A1-3) which are subject to human T cell responses as detected in peripheral blood of melanoma patients by means of IFN-gamma ELISPOT and cytotoxicity assays. In addition, IFN-gamma responses to S100A4 A1-2 can not only be induced by stimulation of T cells with peptide-loaded DC but also by stimulation with S100A4 protein-loaded DC, indicating that this epitope is indeed generated by processing of the endogenously expressed protein. In addition, S100A4 A1-2 reactive T cells demonstrate lysis of HLA-A1(+) fibroblasts in comparison to HLA-A1(-) fibroblasts. In summary, this HLA-A1-restricted peptide epitope is a candidate for immunotherapeutical approaches targeting S100A4-expressing cells in the tumor stroma.
- Find related publications in this database (using NLM MeSH Indexing)
- Amino Acid Sequence -
- Cell Line, Tumor -
- Cells, Cultured -
- Epitopes - immunology Epitopes - metabolism
- HLA-A1 Antigen - immunology
- Humans -
- Interferon-gamma - biosynthesis Interferon-gamma - immunology
- Melanoma - immunology Melanoma - pathology
- Molecular Sequence Data -
- Peptide Fragments - immunology
- S100 Proteins - immunology
- Sequence Alignment -
- Skin Neoplasms - immunology Skin Neoplasms - pathology
- T-Lymphocytes, Cytotoxic - immunology
- Find related publications in this database (Keywords)
- CTL
- HLA-A1 epitope
- Melanoma
- Metastasin 1
- S100A4
- Tumor stroma