Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Stadlbauer, V; Mookerjee, RP; Hodges, S; Wright, GA; Davies, NA; Jalan, R.
Effect of probiotic treatment on deranged neutrophil function and cytokine responses in patients with compensated alcoholic cirrhosis.
J Hepatol. 2008; 48(6): 945-951.
Doi: 10.1016/j.jhep.2008.02.015
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Stadlbauer-Köllner Vanessa
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- Abstract:
- BACKGROUND/AIM: Endotoxaemia contributes to neutrophil dysfunction, infection risk and mortality in patients with alcoholic cirrhosis. As probiotics may decrease Gram-negative gut organisms, we hypothesised that probiotic treatment would restore neutrophil function. METHODS: In an open-label study, patients with alcoholic cirrhosis (n=12) received Lactobacillus casei Shirota (6.5 x 10(9)) 3 times daily for 4 weeks. Data were compared to healthy controls (n=13) and cirrhotic patients (n=8) who did not receive probiotics. Neutrophil oxidative burst, phagocytosis, toll-like-receptor (TLR) expression, plasma cytokines and ex vivo endotoxin-stimulated cytokine production were measured. RESULTS: Baseline neutrophil phagocytic capacity in patients was significantly lower compared to healthy controls (73% versus 98%, p<0.05), but normalised at the end of the study (n=10, 100%, p<0.05). No improvement was seen in disease controls. Soluble TNF-receptor (sTNFR)-1 and-2 and interleukin (IL)10 were significantly elevated in patients' plasma but did not change during the study. Ex vivo endotoxin-stimulated levels of sTNFR1, sTNFR2 and IL10 were significantly lower at the end of the study (p<0.05). TLR2, 4 and 9 were overexpressed in patients. TLR4 expression normalised by the end of the study. CONCLUSIONS: Our data provide a proof-of-concept that probiotics restore neutrophil phagocytic capacity in cirrhosis, possibly by changing IL10 secretion and TLR4 expression, warranting larger randomised controlled and mechanistic studies.
- Find related publications in this database (using NLM MeSH Indexing)
- Female -
- Humans -
- Interleukin-10 - blood
- Lactobacillus casei - physiology
- Liver Cirrhosis, Alcoholic - blood Liver Cirrhosis, Alcoholic - drug therapy Liver Cirrhosis, Alcoholic - physiopathology
- Male -
- Middle Aged -
- Neutrophils - drug effects Neutrophils - physiology
- Phagocytosis - drug effects
- Probiotics - pharmacology Probiotics - therapeutic use
- Receptors, Tumor Necrosis Factor, Type I - blood
- Receptors, Tumor Necrosis Factor, Type II - blood
- Respiratory Burst - drug effects
- Toll-Like Receptor 2 - blood
- Toll-Like Receptor 4 - blood
- Toll-Like Receptor 9 - blood
- Find related publications in this database (Keywords)
- alcoholic cirrhosis
- neutrophil function
- phagocytosis
- probiotic
- cytokine
- toll-like receptor