Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
BURG, G; KERL, H; KAUDEWITZ, P; BRAUNFALCO, O; MASON, DY.
Immunoenzymatic typing of lymphoplasmacytoid skin infiltrates.
J DERMATOL SURG ONCOL. 1984; 10(4): 284-290.
Doi: 10.1111/j.1524-4725.1984.tb00900.x
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- Co-Autor*innen der Med Uni Graz
- Kerl Helmut
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- Abstract:
- Monoclonal immunoglobulin-producing lymphomas (immunocytomas, plasmacytomas, and immunoblastomas) constitute 15 to 20% of all cutaneous lymphomas. Their differentiation from polymorphous lymphoplasmacytoid inflammatory or pseudolymphomatous infiltrates may be difficult if sections are stained for a single light chain only. It was the aim of the study to elucidate the ratio of the kappa- to lambda-positive cells in 10 lymphoproliferative, 5 pseudolymphomatous, and 42 inflammatory lymphoplasmacytoid cutaneous infiltrates and to characterize them by the type of Ig (alpha, gamma, mu, kappa, or lambda) synthesized intracellularly. An indirect immunoenzymatic double-labeling method (alkaline phosphatase and peroxidase) was used for the simultaneous demonstration of kappa and lambda light chains and alpha, gamma, and mu heavy chains in paraffin sections. Ig-producing lymphomas of the skin show patchy monoclonal proliferations of cells synthesizing kappa IgM in almost 50% of the cases (5 of 10). Monoclonality is claimed if the ratio of lambda- to kappa-positive cells is at least 1:10, or vice versa. In polyclonal inflammatory and pseudolymphomatous infiltrates, the lambda/kappa ratio never exceeds 1:5. The most common cell type found in these reactive infiltrates produces gamma heavy chain; some produce alpha chain, but only a few produce mu chain. In high-grade malignant lymphomas, the tumor cells may lose their capacity for Ig production.
- Find related publications in this database (using NLM MeSH Indexing)
- B-Lymphocytes -
- Humans -
- Immunoglobulin kappa-Chains - analysis
- Immunoglobulin lambda-Chains - analysis
- Lymphoma - enzymology Lymphoma - immunology Lymphoma - pathology
- Plasmacytoma - enzymology Plasmacytoma - immunology Plasmacytoma - pathology
- Skin Neoplasms - enzymology Skin Neoplasms - immunology Skin Neoplasms - pathology