Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Svejda, B; Aguiriano-Moser, V; Sturm, S; Höger, H; Ingolic, E; Siegl, V; Stuppner, H; Pfragner, R.
Anticancer activity of novel plant extracts from Trailliaedoxa gracilis (W. W. Smith and Forrest) in human carcinoid KRJ-I Cells.
Anticancer Res. 2010; 30(1):55-64
Web of Science
PubMed
- Führende Autor*innen der Med Uni Graz
- Pfragner Roswitha
- Co-Autor*innen der Med Uni Graz
- Aguiriano Moser Victor
- Siegl Veronika
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- Abstract:
- Background: Small intestinal (SI) neuroendocrine tumors (NETs) are rare neoplasms derived from neuroendocrine cells presenting distinct clinical symptoms according to the ability to secrete neuroamines. Nevertheless, many arc, asymptomatic and misdiagnosed. As response rates to chemotherapy are low, surgery remains the only effective treatment. Because many tumors have metastasized at the time of diagnosis, curative surgery is rarely achieved. Consequently, a substantial need for new therapeutic options has emerged. Materials and Methods: The effects of novel plant extracts from Trailliaedoxa gracilis (W.W. Smith & Forrest) were investigated in the SI-NET cell line KRJ-I and in KRJ-I transplanted mice. Proliferation and viability were analyzed using cell counting and WST-I cell proliferation assay. Apoptosis was determined by DAPI staining and electron microscopy, and quantified by luminescence assays for caspases 3/7, 6, 8, 9 and 2. Results: Extracts of Trailliaedoxa gracilis showed a dose-dependent reduction of proliferation and induction of apoptosis in the KRJ-I cells. Normal fibroblasts were not impaired. Tumor growth inhibition was also observed in heterotransplanted SCID (severe combined immunodeficiency) mice. Conclusion: The in vitro and in vivo outcomes suggest a potential clinical effect of Trailliaedoxa gracilis in SI-NETs.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Carcinoid Tumor - drug therapy
- Caspase 3 - metabolism
- Caspase 7 - metabolism
- Cell Growth Processes - drug effects
- Cell Line, Tumor -
- Female -
- Fibroblasts - cytology
- Humans -
- Intestinal Neoplasms - drug therapy
- Intestine, Small - pathology
- Mice -
- Mice, SCID -
- Neuroendocrine Tumors - drug therapy
- Plant Extracts - pharmacology
- Rubiaceae - chemistry
- Xenograft Model Antitumor Assays -
- Find related publications in this database (Keywords)
- Neuroendocrine tumor
- carcinoid
- cell lines
- plant extracts
- bioactive agents
- chemoresistance
- apoptosis