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SHR Neuro Cancer Cardio Lipid Metab Microb
Kozma, N; Halasz, M; Palkovics, T; Szekeres-Bartho, J.
The progesterone-induced blocking factor modulates the balance of PKC and intracellular Ca.
Am J Reprod Immunol. 2006; 55(2): 122-129.
Doi: 10.1111/j.1600-0897.2005.00337.x
Web of Science
PubMed
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- Leading authors Med Uni Graz
- Kozma Noemi
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- Abstract:
- PROBLEM: Progesterone-induced blocking factor (PIBF) induces Th2 biased cytokine production; therefore, this study investigates the effects of PIBF on the protein kinase C (PKC)/Ca(++) system - which plays a key role in Th1/Th2 differentiation. METHOD OF STUDY: Proteins from PIBF-treated cells were reacted on Western blots with phospho-specific antibodies recognizing different PKC izoforms. Intracellular free calcium was measured by flow cytometry. RESULTS: Both interleukin (IL)-4 and PIBF induced PKC phosphorylation, which was abrogated by anti-IL-4Ralpha or anti-PIBF immunoglobulin G pre-treatment. PIBF treatment did not alter intracellular Ca(++) levels. Inhibition of PKCzeta or PKCtheta phosphorylation, but not that of PKCalpha/beta resulted in the loss of STAT6 and Jak1 phosphorylation by PIBF. CONCLUSIONS: Our data show that PIBF phosphorylates PKC via binding to the IL-4R, without affecting intracellular Ca(++). Phosphorylation of PKCzeta and PKCtheta is required for Jak1 and STAT6 activation, whereas PKCalpha/beta is not involved. These findings explain the mechanism by which PIBF supports a Th2 dominant cytokine pattern.
- Find related publications in this database (using NLM MeSH Indexing)
- Calcium - metabolism
- Female -
- Humans -
- Janus Kinase 1 -
- Lymphocytes - metabolism
- Models, Biological -
- Phosphorylation -
- Pregnancy Proteins - physiology
- Protein Kinase C - metabolism
- Protein-Tyrosine Kinases - metabolism
- Receptors, Interleukin-4 - chemistry
- STAT6 Transcription Factor - metabolism
- Suppressor Factors, Immunologic -
- Find related publications in this database (Keywords)
- cytokine
- signaling
- Th2