Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Lindpointner, S; Korsatko, S; Köhler, G; Köhler, H; Schaller, R; Schaupp, L; Ellmerer, M; Pieber, TR; Regittnig, W.
Glucose levels at the site of subcutaneous insulin administration and their relationship to plasma levels.
Diabetes Care. 2010; 33(4): 833-838.
Doi: 10.2337/dc09-1531
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- Leading authors Med Uni Graz
- Lindpointner Stefan
- Regittnig Werner
- Co-authors Med Uni Graz
- Ellmerer Martin
- Köhler Gerd
- Korsatko Stefan
- Pieber Thomas
- Schaupp Lukas
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- Abstract:
- OBJECTIVE - To examine insulin's effect on the tissue glucose concentration at the site of subcutaneous insulin administration. RESEARCH DESIGN AND METHODS - A CMA-60 microdialysis (MD) catheter and a 24-gauge microperfusion (MP) catheter were inserted into the subcutaneous adipose tissue of fasting, healthy subjects (n = 5). Both catheters were perfused with regular human insulin (100 units/ml) over a 6-h period and used for glucose sampling and simultaneous administration of insulin at sequential rates of 0.33, 0.66, and 1.00 units/h (each rate was used for 2 h). Before and after the insulin delivery period, both catheters were perfused with an insulin-free solution (5% mannitol) for 2 h and used for glucose sampling only. Blood plasma glucose was clamped at euglycemic levels during insulin delivery. RESULTS - Start of insulin delivery with MD and MP catheters resulted in a decline of the tissue glucose concentration and the tissue-to-plasma glucose ratio (TPR) for similar to 60 min (P < 0.05). However, during the rest of the 6-h period of variable insulin delivery, tissue glucose concentration paralleled the plasma glucose concentration, and the TPR for MD and MP catheters remained unchanged at 83.2 +/- 3.1 and 77.1 +/- 4.8%, respectively. After subsequent switch to insulin-free perfusate, tissue glucose concentration and TPR increased slowly and reattained preinsulin delivery levels by the end of the experiments. CONCLUSIONS - The results show the attainment of a stable TPR value at the site of insulin administration, thus indicating that insulin delivery and glucose sensing may be performed simultaneously at the same adipose tissue site.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Blood Glucose - drug effects
- Glucose - metabolism
- Humans -
- Hypoglycemic Agents - administration and dosage
- Infusions, Subcutaneous -
- Insulin - administration and dosage
- Male -
- Models, Theoretical -
- Subcutaneous Tissue - metabolism