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SHR Neuro Cancer Cardio Lipid Metab Microb
Raggam, RB; Wagner, J; Bozic, M; Michelin, BD; Hammerschmidt, S; Homberg, C; Kessler, HH.
Detection and quantitation of Epstein-Barr virus (EBV) DNA in EDTA whole blood samples using automated sample preparation and real time PCR.
Clin Chem Lab Med. 2010; 48(3): 413-418.
Doi: 10.1515/CCLM.2010.064
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Kessler Harald
- Raggam Reinhard Bernd
- Co-authors Med Uni Graz
- Bozic Michael
- Rabensteiner Jasmin
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- Abstract:
- Background: Detection and quantitation of Epstein-Barr virus (EBV) DNA in EDTA whole blood samples has gained significance in the routine diagnostic laboratory. Methods: In this study, the analytical and clinical performance of the artus (R) EBV RG PCR kit in conjunction with automated sample preparation on the QIAsymphony (R) SP instrument was evaluated. Results: When the accuracy of the new test system was tested, all results were found to be within +/- 0.5 log(10) unit of the expected panel results. Determination of linearity showed a quasilinear curve over 4 log units. The lower limit of detection was determined to be 391 EBV DNA copies/mL in EDTA whole blood. The between day imprecision ranged from 18% to 66%, and the within run imprecision ranged from 11% to 50%. When clinical samples were tested and the results compared with those obtained with the routinely used easyMAG (TM) sample preparation and EBV R-gene (TM) test system, 60 samples tested positive and 31 samples tested negative by both assays. Nineteen samples were found to be positive using the QIAsymphony (R) sample preparation and artus (R) EBV RG PCR test system only, and no samples tested positive with the routinely used test system only. Conclusions: The QIAsymphony (R) sample preparation and artus (R) EBV RG PCR test system is suitable for the detection and quantitation of EBV DNA in EDTA whole blood in the routine diagnostic laboratory. Clin Chem Lab Med 2010;48:413-8.
- Find related publications in this database (using NLM MeSH Indexing)
- Automation -
- DNA, Viral - blood
- Edetic Acid - chemistry
- Epstein-Barr Virus Infections - diagnosis
- Herpesvirus 4, Human - genetics Herpesvirus 4, Human - isolation and purification
- Humans -
- Polymerase Chain Reaction - methods
- Reagent Kits, Diagnostic -
- Reproducibility of Results -
- Find related publications in this database (Keywords)
- Epstein-Barr virus (EBV)
- molecular diagnostics
- quantitation
- real-time PCR
- sample preparation