Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Erlacher, L; Maier, R; Ullrich, R; Kiener, H; Aringer, M; Menschik, M; Graninger, W.
Differential expression of the protooncogene bcl-2 in normal and osteoarthritic human articular cartilage.
J Rheumatol. 1995; 22(5):926-931
Web of Science
PubMed
- Führende Autor*innen der Med Uni Graz
- Graninger Winfried
- Co-Autor*innen der Med Uni Graz
- Graninger Monika
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- Abstract:
- OBJECTIVE. To investigate the expression of bcl-2 in healthy and osteoarthritic articular cartilage at the transcriptional level and at the protein level. METHODS. bcl-2 mRNA was detected by reverse transcriptase/polymerase chain reaction. The expression of bcl-2 protein was studied using flow cytometry analysis with a monoclonal antibody against intracellular bcl-2 and by immunohistochemistry. RESULTS. mRNA for bcl-2 was detectable in chondrocytes of both healthy and osteoarthritic cartilage. bcl-2 protein was present in the chondrocytes of the middle layer of cartilage by immunohistochemical staining. In osteoarthritis (OA), chondrocytes adjacent to cartilage defects expressed a particularly high signal intensity. Flow cytometry analysis demonstrated the presence of bcl-2 protein in virtually all chondrocytes. CONCLUSION. Our results show that bcl-2 is expressed in healthy adult and osteoarthritic human cartilage. The increased staining of chondrocytes adjacent to osteoarthritic defects indicates a differential regulation of programmed cell death during the degenerative process in OA.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Aged -
- Base Sequence -
- Cartilage, Articular - chemistry
- Cell Death - genetics
- Female -
- Flow Cytometry -
- Gene Expression -
- Humans -
- Male -
- Middle Aged -
- Molecular Sequence Data -
- Osteoarthritis - genetics Osteoarthritis - pathology
- Polymerase Chain Reaction -
- Proto-Oncogene Proteins - analysis
- Proto-Oncogene Proteins c-bcl-2 -
- Proto-Oncogenes - genetics
- RNA, Messenger - analysis
- Find related publications in this database (Keywords)
- BCL-2
- PROTOONCOGENE
- CARTILAGE
- OSTEOARTHRITIS
- APOPTOSIS