Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Schicher, M; Kollroser, M; Hermetter, A.
Mapping the lipolytic proteome of adipose tissue using fluorescent suicide inhibitors.
Methods Mol Biol. 2009; 579: 497-511.
Doi: 10.1007/978-1-60761-322-0_25
PubMed
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- Co-authors Med Uni Graz
- Kollroser Manfred
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- Abstract:
- Lipases are responsible for the hydrolysis of acylglycerols and cholesteryl esters in animals, plants, and microorganisms. In this chapter we describe a tool for the concomitant analysis of lipases in complex proteomes. For this purpose, the target enzymes are selectively and covalently labelled with fluorescent suicide inhibitors. Stable lipid-protein complexes are formed that are separated by gel electrophoresis and identified by mass spectrometry.
- Find related publications in this database (using NLM MeSH Indexing)
- Adipose Tissue - chemistry
- Alkylation -
- Animals -
- Chromatography, Liquid -
- Electrophoresis, Polyacrylamide Gel -
- Fluorescent Dyes - chemistry
- Isoelectric Focusing -
- Lipase - metabolism
- Lipids - chemistry
- Mice -
- Oxidation-Reduction -
- Proteome -
- Tandem Mass Spectrometry -