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SHR Neuro Cancer Cardio Lipid Metab Microb

Liu, ZY; Obenauf, AC; Speicher, MR; Kopan, R.
Rapid identification of homologous recombinants and determination of gene copy number with reference/query pyrosequencing (RQPS).
GENOME RES. 2009; 19(11): 2081-2089. Doi: 10.1101/gr.093856.109 [OPEN ACCESS]
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Co-authors Med Uni Graz: Obenauf Anna Christina; Speicher Michael
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Abstract:: Manipulating the mouse genome is a widespread technology with important applications in many biological fields ranging from cancer research to developmental biology. Likewise, correlations between copy number variations (CNVs) and human diseases are emerging. We have developed the reference-query pyrosequencing (RQPS) method, which is based on quantitative pyrosequencing and uniquely designed probes containing single nucleotide variations (SNVs), to offer a simple and affordable genotyping solution capable of identifying homologous recombinants independent of the homology arm size, determining the micro-amplification status of endogenous human loci, and quantifying virus/transgene copy number in experimental or commercial species. In addition, we also present a simple pyrosequencing-based protocol that could be used for the enrichment of homologous recombinant embryonic stem (ES) cells.
Find related publications in this database (using NLM MeSH Indexing): Algorithms -; Animals -; Embryonic Stem Cells - metabolism; Gene Dosage -; Gene Frequency -; Genetic Predisposition to Disease - genetics; Genomics - methods; Genotype -; Humans -; Mice -; Mice, Inbred C57BL -; Mice, Inbred Strains -; Mice, Transgenic -; Phosphoglycerate Kinase - genetics; Point Mutation -; Polymorphism, Single Nucleotide -; Receptor, Notch2 - genetics; Recombination, Genetic -; Reproducibility of Results -; Sequence Analysis, DNA - methods; Transgenes - genetics