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SHR Neuro Cancer Cardio Lipid Metab Microb
Lamprecht, G; Stoschitzky, K.
Enantioselective analysis of R- and S-propafenone in plasma by HPLC applying column switching and liquid-liquid extraction
J CHROMATOGR B. 2009; 877(29): 3489-3494.
Doi: 10.1016/j.jchromb.2009.08.024
Web of Science
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- Co-authors Med Uni Graz
- Stoschitzky Kurt
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- Abstract:
- Two HPLC methods are described for the enantioselective analysis of R- and S-propafenone in plasma. In a Column switching approach, centrifuged plasma was injected onto a silica-based strong acid cation-exchanger and the fraction containing propafenone was switched on-line onto an enantioselective Chiralcel column for separation of the enantiomers. In another approach, propafenone was extracted from plasma by liquid-liquid extraction at pH 11.4. The extracted components were transferred into aqueous medium and separated on a Chiralcel ODR. Both methods were validated and showed comparable performance. Within-day and between-day precision was better than 5% for both methods. Linear calibration functions were obtained (r(2) > 0.999), and the limit of detection for each enantiomer was 0.2 mu g/mL for column switching and 0.55 mu g/mL for liquid-liquid extraction. The analysis methods were applied for the determination of the effect of physical exercise on the enantiomeric ratio of R- and S-propafenone in plasma of healthy volunteers. During exercise, the concentration of both enantiomers reached a maximum, followed by a significant decrease during recovery. (C) 2009 Elsevier B.V. All rights reserved.
- Find related publications in this database (Keywords)
- Propafenone
- Enantioselective analysis
- HPLC
- Column switching
- Liquid-liquid extraction