Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Rabensteiner, DF; Spreitzhofer, E; Kirchengast, S; Wachswender, C; Horwath-Winter, J; Schmut, O.
Die Verweildauer von Pollen an der Augenoberfläche - erste Beobachtungen in-vivo
Spektrum der Augenheilkunde. 2009; 23(5): 343-346.
Doi: 10.1007/s00717-009-0353-6
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- Führende Autor*innen der Med Uni Graz
- Rabensteiner Dieter Franz
- Co-Autor*innen der Med Uni Graz
- Horwath-Winter Jutta
- Kirchengast Sieglinde
- Schmut Otto
- Wachswender Christine
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- Abstract:
- BACKGROUND: In our laboratory biochemical analysts prepared birch pollen (Betula pendula), a well known aggressive pollen species, for different kinds of studies. Hours after pollen exposure the analysts still suffered from ocular and nasal sensations of dryness. We investigated the residence time of pollen on the ocular surface in-vivo. METHODS: Two biochemical analysts prepared birch pollen in our laboratory as usual. At a working distance of about 40 cm each analyst prepared 50 mg of pollen for 30 minutes. The dried male inflorescences which contain pollen were separated, sieved and discharged at room-temperature (20A degrees C). Immediately afterwards, 10 minutes later and after 3 and 5 hours, respectively, a smear in the area of the medial lower fornix was taken from the conjunctiva using a plastic loop. The smear was transferred to a slide and examined with an optical microscope. After the first smear one of the eyes of each biochemical analyst was rinsed with 0.25% Na-hyaluronate for four times. RESULTS: Immediately after the preparation of pollen, 10 minutes later and 3 and 5 hours, respectively, after pollen exposure, pollen grains could be found in the conjunctival smear. After 10 minutes only a little amount of pollen was left in the smears of the rinsed eyes. A decline of the number of pollen grains could be observed. Again the biochemical analysts declared ocular and nasal sensations of dryness that lasted for several hours. CONCLUSION: Besides their allergenic potential recent studies have proven that pollen can destroy human tear fluid and damage human conjunctival cells by protease reaction, provoking the seasonal non-allergic conjunctivitis (SNAC). Our observations show that pollen have sufficient time to promote damage by protease reaction before being swept away by lacrimation.
- Find related publications in this database (Keywords)
- Pollen
- residence time
- ocular surface
- in-vivo examination