Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Weissenböck, H; Mrakovcic, M; Ladinig, A; Fragner, K.
In situ hybridization for Lawsonia intracellularis--specific 16s rRNA sequence in paraffin-embedded tissue using a digoxigenin-labeled oligonucleotide probe.
J Vet Diagn Invest. 2007; 19(3): 282-285.
Doi: 10.1177/104063870701900309
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Co-authors Med Uni Graz
- Fröhlich Maria
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- An in situ hybridization (ISH) procedure with a digoxigenin-labeled oligonucleotide probe for detection of Lawsonia intracellularis in paraffin-embedded tissue is described. This technique recognized 71% of PCR-positive cases and was thus superior to Warthin-Starry silver stain, which only detected 41%. The presented ISH is of comparable sensitivity to previously published immunohistochemical assays and is recommended for laboratories wishing to diagnose L. intracellularis infections in tissue sections but without access to antibodies.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- DNA, Bacterial - genetics
- Desulfovibrionaceae Infections - diagnosis Desulfovibrionaceae Infections - microbiology Desulfovibrionaceae Infections - veterinary
- Digoxigenin -
- In Situ Hybridization - methods In Situ Hybridization - veterinary
- Intestinal Diseases - diagnosis Intestinal Diseases - microbiology Intestinal Diseases - veterinary
- Lawsonia Bacteria - genetics Lawsonia Bacteria - isolation and purification
- Oligonucleotide Probes - genetics
- Paraffin Embedding -
- RNA, Ribosomal, 16S - genetics
- Sensitivity and Specificity -
- Swine -
- Swine Diseases - diagnosis Swine Diseases - microbiology
- Find related publications in this database (Keywords)
- in situ hybridization
- Lawsonia intracellularis
- oligonucleotide probe
- porcine proliferative enteropathy