Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Schildhauer, TA; Chapman, JR; Muhr, G; Koller, M.
Cytokine release of mononuclear leukocytes (PBMC) after contact to a carbonated calcium phosphate bone cement
J BIOMED MATER RES PART A. 2006; 78A(1): 104-109.
Doi: 10.1002/jbm.a.30784
Web of Science
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Schildhauer Thomas Armin
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Human leukocytes (peripheral blood mononuclear cells, PBMC) were overlaid on calcium phosphate bone cement (CBC, Norian SRS (R)) and allowed to settle for 1 h under cell culture conditions. Subsequently, the cells were either left unstimulated (i.e. sham stimulation using cell culture medium), or stimulated with toxic shock syndrome toxin-1 (TSST-1, 10 ng/mL), staphylococcal enterotoxin B (SEB, 10 ng/mL), or concanavalin A (ConA, 2 mu g/mL) for further 24 h using cell culture conditions. Supernatants were then analyzed for cytokine content (interleukin-1 receptor antagonist, IL-1ra; IL-2; IL-6; IL-10; IL-12) by enzyme-linked immunosorbent assay. While the spontaneous generation of cytokines was not influenced, the IL-2 release from stimulated PBMC was significantly decreased in contrast to other analyzed cytokines after contact to the curing CBC compared to control incubations without CBC. This decrease in IL-2 release was not due to known inhibitors of IL-2 synthesis platelet factor-4 (PF-4), IL-10, TGF-beta, or elevated calcium ion concentrations. (c) 2006 Wiley Periodicals, Inc.
- Find related publications in this database (Keywords)
- calcium phosphate cement
- T-cell
- interleukin
- leukocyte
- suppression