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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Schwach, G; Tschemmernegg, M; Pfragner, R; Ingolic, E; Schreiner, E; Windisch, M.
Establishment of stably transfected rat neuronal cell lines expressing alpha-synuclein GFP fusion proteins.
J Mol Neurosci. 2010; 41(1): 80-88.
Doi: 10.1007/s12031-009-9289-1
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PubMed
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- Leading authors Med Uni Graz
- Pfragner Roswitha
- Schwach Gert
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- Abstract:
- Mutations in the alpha-synuclein gene have been linked to rare cases of familial Parkinson's disease (PD). alpha-Synuclein, a 140 amino acid polypeptide, is a major component of Lewy bodies (LB), a pathological hallmark of PD. Transgenic mice, Drosophila and marmosets (Challitrix jacchus) expressing either wild type (WT) or mutant human alpha-synuclein develop motor deficits, LB-like inclusions in some neurons and neuronal degeneration. The effects of human alpha-synuclein were investigated in a neuronal rat cell line (B103). Plasmids expressing WT and mutant human alpha-synuclein regulated by the cytomegalovirus (CMV) promoter were prepared and used for creating stably transfected neuronal rat cell lines. For localizing alpha-synuclein expression, stably transfected neuronal rat cell lines, expressing alpha-synuclein enhanced green fluorescent protein fusion proteins, regulated by either the CMV or the human platelet-derived growth factor ss promoter were generated. Over-expression of WT and A53T alpha-synuclein regulated by CMV promoter in stable transfectants resulted in formation of alpha-synuclein-immunopositive inclusion-like structures and mitochondrial alterations. Taken together, these results suggest that abnormal accumulation of alpha-synuclein could lead to mitochondrial alterations that might result in oxidative stress and eventually, cell death.
- Find related publications in this database (using NLM MeSH Indexing)
- Aged -
- Animals -
- Cell Line - physiology Cell Line - ultrastructure
- Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism
- Humans -
- Mice -
- Neurons - physiology Neurons - ultrastructure
- Parkinson Disease - pathology Parkinson Disease - physiopathology
- Rats -
- Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism
- Transfection -
- alpha-Synuclein - genetics alpha-Synuclein - metabolism
- Find related publications in this database (Keywords)
- alpha-Synuclein
- EGFP
- Parkinson's disease
- B 103 cell line