Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Raffeiner, B; Dejaco, C; Duftner, C; Kullich, W; Goldberger, C; Vega, SC; Keller, M; Grubeck-Loebenstein, B; Schirmer, M.
Between adaptive and innate immunity: TLR4-mediated perforin production by CD28null T-helper cells in ankylosing spondylitis.
Arthritis Res Ther. 2005; 7(6): R1412-R1420.
Doi: 10.1186/ar1840
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- Führende Autor*innen der Med Uni Graz
- Dejaco Christian
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- Abstract:
- CD3+CD4+CD28null and CD3+CD8+CD28null T cells are enriched in patients with immune-mediated diseases compared with healthy controls. This study shows that CD4+CD28null T cells express Toll-like receptors recognizing bacterial lipopolysaccharides in ankylosing spondylitis, psoriatic arthritis and rheumatoid arthritis. In ankylosing spondylitis, TLR4 (23.1 +/- 21.9%) and, to a smaller extent, TLR2 (4.1 +/- 5.8%) were expressed on CD4+CD28null T cells, whereas expression was negligible on CD4+CD28+ and CD8+ T cells. CD4+CD28null T cells produced perforin upon stimulation with lipopolysaccharide, and this effect was enhanced by autologous serum or recombinant soluble CD14. Perforin production could be prevented with blocking antibodies directed against CD14 or TLR4. Incubation of peripheral blood mononuclear cells with tumour necrosis factor alpha led to an upregulation of TLR4 and TLR2 on CD4+CD28null T cells in vitro, and treatment of patients with antibodies specifically directed against tumour necrosis factor alpha resulted in decreased expression of TLR4 and TLR2 on CD4+CD28null T cells in vivo. We describe here a new pathway for direct activation of cytotoxic CD4+ T cells by components of infectious pathogens. This finding supports the hypothesis that CD4+CD28null T cells represent an immunological link between the innate immune system and the adaptive immune system.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Antibodies, Blocking - pharmacology
- Antigens, CD28 - metabolism
- Arthritis, Psoriatic - immunology Arthritis, Psoriatic - metabolism Arthritis, Psoriatic - pathology
- Arthritis, Rheumatoid - immunology Arthritis, Rheumatoid - metabolism Arthritis, Rheumatoid - pathology
- CD4-Positive T-Lymphocytes - immunology CD4-Positive T-Lymphocytes - metabolism CD4-Positive T-Lymphocytes - pathology
- Cell Line -
- Female -
- Humans -
- Immunity - immunology
- Leukocytes, Mononuclear - drug effects Leukocytes, Mononuclear - metabolism Leukocytes, Mononuclear - pathology
- Lipopolysaccharides - pharmacology
- Male -
- Membrane Glycoproteins - genetics Membrane Glycoproteins - metabolism
- Middle Aged -
- Perforin -
- Pore Forming Cytotoxic Proteins -
- RNA, Messenger - metabolism
- Spondylitis, Ankylosing - immunology Spondylitis, Ankylosing - metabolism Spondylitis, Ankylosing - pathology
- T-Lymphocytes, Helper-Inducer - drug effects T-Lymphocytes, Helper-Inducer - metabolism T-Lymphocytes, Helper-Inducer - pathology
- Toll-Like Receptor 4 - genetics Toll-Like Receptor 4 - metabolism
- Tumor Necrosis Factor-alpha - pharmacology