Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Zimmermann, R; Sartipy, P; Winkler, R; Zechner, R; Hurt-Camejo, E; Kostner, GM.
Endogenously produced glycosaminoglycans affecting the release of lipoprotein lipase from macrophages and the interaction with lipoproteins.
Biochim Biophys Acta. 2000; 1484(2-3):316-324
Doi: 10.1016/S1388-1981(00)00024-X
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- Führende Autor*innen der Med Uni Graz
- Kostner Gerhard
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- Abstract:
- Macrophages are intimately involved in the pathogenesis of atherosclerotic diseases. A key feature of this process is their uptake of various lipoproteins and subsequent transformation to foam cells. Since lipoprotein lipase (LPL) is believed to play a role in foam cell formation, we investigated if endogenously produced proteoglycans (PGs) affect the release of this enzyme from macrophages. The human leukaemic cell line THP-1 which differentiates into macrophages by treatment with phorbol ester (phorbol 12-myristate 13-acetate) served as a model. The differentiation of THP-1 macrophages promoted the release of PGs into the cell medium which caused the detachment of LPL activity from the cell surface, and prevented LPL re-uptake and inactivation. These PGs were mainly composed of chondroitin sulfate type and exerted a heparin-like effect on LPL release. LPL is known to increase the cell association of lipoproteins by the well known bridging function. Exogenous bovine LPL at a concentration of 1 microg/ml enhanced low density lipoprotein (LDL)-binding 10-fold. Endogenously produced PGs reduced LPL-mediated binding of LDL. It is proposed that the differentiation-dependent increase in the release of PGs interferes with binding of LPL and reduces lipoprotein-binding to macrophages.
- Find related publications in this database (using NLM MeSH Indexing)
- Arteriosclerosis - metabolism
- Cell Line - metabolism
- Chondroitin Sulfates - metabolism
- Culture Media, Conditioned - metabolism
- Dermatan Sulfate - metabolism
- Glycosaminoglycans - biosynthesis
- Heparin - pharmacology
- Humans - pharmacology
- Lipoprotein Lipase - metabolism
- Lipoproteins - metabolism
- Lipoproteins, LDL - metabolism
- Macrophages - metabolism
- Proteoglycans - biosynthesis
- Find related publications in this database (Keywords)
- atherosclerosis
- macrophage
- proteoglycan
- heparin
- low density lipoprotein