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SHR Neuro Cancer Cardio Lipid Metab Microb
Troppan, KT; Stelzl, E; Violan, D; Winkler, M; Kessler, HH.
Evaluation of the new VERSANT HIV-1 RNA 1.0 Assay (kPCR) for quantitative detection of human immunodeficiency virus type 1 RNA.
J CLIN VIROL. 2009; 46(1): 69-74.
Doi: 10.1016/j.jcv.2009.06.012
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Prochazka Katharina
- Co-authors Med Uni Graz
- Kessler Harald
- Stelzl Evelyn
- Winkler Michaela
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- Abstract:
- BACKGROUND: The VERSANT HIV-1 RNA 1.0 Assay (kPCR) for quantitative detection of HIV-1 RNA has recently been introduced. OBJECTIVES: In this study, the performance of the VERSANT HIV-1 RNA 1.0 Assay (kPCR) was evaluated and compared to the COBAS AmpliPrep/COBAS TaqMan HIV-1 Test, version 2.0. STUDY DESIGN: Accuracy, linearity, interassay and intra-assay variations were determined, and a total of 196 routine clinical samples including a high number of HIV-1 subtype non-B samples were investigated. RESULTS: When accuracy of the new kit was tested, all of the quantifiable results were found to be within -0.5log(10) unit of the expected panel results. Determination of linearity resulted in a quasilinear curve up to the initial concentration of 3.4x10(5)copies/mL. The interassay variation ranged from 12 to 20%, and the intra-assay variation ranged from 8 to 16%. When clinical samples were tested by the VERSANT HIV-1 RNA 1.0 Assay (kPCR) and the results were compared with those obtained by the COBAS AmpliPrep/COBAS TaqMan HIV-1 test, version 2.0, the results for 95% of all samples with positive results by both tests were found to be within +/-1.0log(10) unit. The viral loads for all samples measured by the Siemens and Roche assays showed a high correlation (R(2)=0.94); quantitative results obtained by the Siemens assay were usually found to be lower than those obtained by the Roche assay. CONCLUSIONS: The new VERSANT HIV-1 RNA 1.0 Assay (kPCR) proved to be suitable for use in the routine diagnostic laboratory. The time to results was similar for both of the assays.
- Find related publications in this database (using NLM MeSH Indexing)
- HIV-1 - genetics HIV-1 - isolation and purification
- Humans -
- RNA, Viral - genetics RNA, Viral - isolation and purification
- Reagent Kits, Diagnostic -
- Reproducibility of Results -
- Reverse Transcriptase Polymerase Chain Reaction - methods
- Sensitivity and Specificity -
- Viral Load - methods
- Find related publications in this database (Keywords)
- Human immunodeficiency virus type 1
- HIV-1 subtype
- Real-time PCR
- Automation
- VERSANT HIV-1 RNA 1.0 Assay (kPCR)
- COBAS AmpliPrep/COBAS TaqMan HIV-1
- Test, version 2.0