Medizinische Universität Graz - Research portal
Selected Publication:
Malle, E; Rotheneder, M; Vetterlein, M; Kostner, GM.
Influence of morphology and malignancy of three new human melanoma cell lines on the cyclooxygenase pathway.
PROSTAGLANDINS 1989 37: 389-398.
Doi: 10.1016/0090-6980(89)90009-9
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- Leading authors Med Uni Graz
- Malle Ernst
- Co-authors Med Uni Graz
- Dieber-Rotheneder Martina
- Kostner Gerhard
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- Abstract:
- Three newly established human melanoma cell lines (WU-BI, PN-JC, MJ-ZJ) of different morphology and different stage of malignancy were incubated with ionophore A23187 (2.5 to 40 microM) or arachidonic acid (AA, 6.25 to 100 microM). PGF2 alpha, 6-keto-PGF1 alpha, PGE2, TXB2 and 2,3-dinor-TXB2 from isolated cells and supernatants were measured by negative ion chemical ionization gas chromatography/mass spectrometry (GC/MS). PGE2 decreased in the fibroblastoid MJ-ZJ cells from 36.7 ng/mg cell protein about 70% (A23187) and about 20% (AA), respectively. However, in the cell supernatant PGE2 increased up to 295.4 +/- 66.5 ng/mg cell protein. Production of PGF2 alpha and PGE2 increased up to 5.7 +/- 1.2 ng/mg cell protein for polydendritic WU-BI cells and spindle shaped PN-JC cells. Up to 9.3 +/- 4.3 ng PGF2 alpha and 13.4 +/- 4.7 ng PGE2 was measured for WU-BI and PN-JC in the cell supernatants. All three melanoma cell lines completely lacked formation of 6-keto-PGF1 alpha, TXB2, and 2,3-dinor-TXB2.
- Find related publications in this database (using NLM MeSH Indexing)
- Arachidonic Acids - metabolism
- Calcimycin - pharmacology
- Cell Line - pharmacology
- Gas Chromatography-Mass Spectrometry - pharmacology
- Humans - pharmacology
- Melanoma - enzymology
- Prostaglandin-Endoperoxide Synthases - metabolism