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Lindner, W; Rath, M; Stoschitzky, K; Uray, G.
Enantioselective drug monitoring of (R)- and (S)- propranolol in human plasma via derivatization with optically active (R,R)-O,O-diacetyl tartaric acid anhydride.
J Chromatogr. 1989; 487(2):375-383 Doi: 10.1016/S0378-4347(00)83045-5
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Co-authors Med Uni Graz: Stoschitzky Kurt
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Abstract:: A sensitive high-performance liquid chromatographic method was developed for the stereoselective assay of (R)- and (S)-propranolol in human plasma. The method involves diethyl ether extraction of the drugs and a racemic internal standard, N-tert.-butylpropranolol, followed by derivatization of the compounds with the chiral reagent (R,R)-O,O-diacetyl tartaric acid anhydride. The resulting diastereomeric derivatives were separated isocratically on a reversed-phase column. Quantitation was achieved by the peak-height ratio method with reference to the internal standard. The assay was accurate and reproducible in the concentration range 1-100 ng of (R)- and (S)-propranolol per ml plasma, using fluorescence detection at lambda ex 290 nm and lambda em 335 nm. The applicability of this method was demonstrated for the determination of concentration-time profiles of propranolol enantiomers in the course of comparative pharmacokinetic studies.
Find related publications in this database (using NLM MeSH Indexing): Administration, Oral -; Anhydrides - diagnostic use; Female - diagnostic use; Humans - diagnostic use; Indicators and Reagents - diagnostic use; Male - diagnostic use; Propranolol - administration and dosage; Stereoisomerism - administration and dosage; Tartrates - diagnostic use