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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Siddique, ZL; Drozdov, I; Floch, J; Gustafsson, BI; Stunes, K; Pfragner, R; Kidd, M; Modlin, IM.
KRJ-I and BON cell lines: defining an appropriate enterochromaffin cell neuroendocrine tumor model.
NEUROENDOCRINOLOGY. 2009; 89(4): 458-470.
Doi: 10.1159/000209330
Web of Science
PubMed
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- Co-authors Med Uni Graz
- Pfragner Roswitha
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- Abstract:
- Background: Neuroendocrine tumors (NETs) of the gastrointestinal (GI) system are increasing in incidence with minimal improvement in prognosis. Although the cell of origin has been identified as the enterochromaffin (EC) cell, its secretory and proliferative regulation has not been defined at a mechanistic level. To date, the BON cell line has been the most widely used in vitro EC cell model despite its pancreatic origin. Using whole-genome mathematical analysis as well as secretory and proliferative studies, we compared the BON cell line to the small intestine ( SI) EC cell-derived NET cell line, KRJ-I, to assess individual cell line validity and applicability for the investigation of GI-NET disease. Methods and Results: Principal component analysis and ANOVA of KRJ-I and BON transcriptomes (U133 Plus 2) identified substantially different (<10%) overlap in transcripts with minimal (R-2 = 0.24) correlation in gene expression profiles. RTPCR detected large variability (>12%) in neuroendocrine ( NE) marker transcripts in the BON cell line and the absence of Tph-2, DDC, TGF beta R2, and M3 transcripts in KRJ-I. The KRJ-I cell line secreted serotonin (5-HT) in response to isoproterenol (EC50 = 100 nM), noradrenaline (EC50 = 1.7 nM), and pituitary adenylate cyclase (PACAP, EC50 = 0.03 nM). Cholecystokinin (IC50 = 430 nM), somatostatin (IC50 = 400 nM), acetylcholine (IC50 = 3.7 nM), and gamma-aminobutyric acid A (GABA(A), IC50 = 2 nM) all inhibited 5-HT release, while gastrin and bombesin had no effect. 5-HT secretion in the BON cell line was stimulated by isoproterenol (EC50 = 900 nM), noradrenaline (EC50 = 20 nM), cholecystokinin (EC50 = 130 nM), PACAP (EC50 = 0.12 nM), bombesin (EC50 = 15 nM), and acetylcholine (EC50 = 0.2 nM). It was inhibited by somatostatin (IC50 = 300 nM) but not GABA A. KRJ-I responded with proliferation to connective tissue growth factor (CTGF, EC50 = 0.002 ng/ml), transforming growth factor-alpha (TGF alpha, EC50 = 0.63 ng/ml) and transforming growth factor-beta (TGF beta, EC50 = 0.63 ng/ml). Epidermal growth factor (EGF) and somatostatin had no significant effect. BON cell proliferation was stimulated only by EGF and TGF alpha (EC50 = 15.8 and 10 ng/ml). TGF beta (IC50 = 0.16 ng/ml), MZ-4-147 (IC50 = 0.5 nM), and BIM23A761 (IC50 = 0.06 nM) all inhibited proliferation. CTGF and somatostatin had no effect. Conclusion: KRJ-I and BON cell lines demonstrate substantial differences in gene level transcripts, inconsistent receptor profile expression, wide variability in NE marker transcript levels, and significantly differential proliferative and secretory responses. Given the EC cell origin of KRJ-I, these results provide evidence that the BON cell line does not represent an EC cell system and is not a valid study model of (carcinoid) EC cell-derived NET. Copyright (C) 2009 S. Karger AG, Basel
- Find related publications in this database (using NLM MeSH Indexing)
- Cell Line, Tumor -
- Dose-Response Relationship, Drug -
- Enterochromaffin Cells - drug effects
- Growth Inhibitors - pharmacology
- Humans -
- Intercellular Signaling Peptides and Proteins - pharmacology
- Neuroendocrine Tumors - drug therapy
- Find related publications in this database (Keywords)
- Neuroendocrine tumors
- Enterochromaffin cell
- BON cell line
- KRJ-I cell line