Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Bachleitner-Hofmann, T; Friedl, J; Hassler, M; Hayden, H; Dubsky, P; Sachet, M; Rieder, E; Pfragner, R; Brostjan, C; Riss, S; Niederle, B; Gnant, M; Stift, A.
Pilot trial of autologous dendritic cells loaded with tumor lysate(s) from allogeneic tumor cell lines in patients with metastatic medullary thyroid carcinoma.
ONCOL REP. 2009; 21(6): 1585-1592.
Doi: 10.3892/or_00000391
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Co-authors Med Uni Graz
- Pfragner Roswitha
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Immunotherapy with autologous dendritic cells (DCs) loaded with tumor lysate(s) from allogeneic tumor cell lines is a novel strategy to induce immune responses in cancer patients. We report on a pilot trial of autologous DCs pulsed with tumor cell lysate derived from allogeneic medullary thyroid carcinoma (MTC) cell lines in patients with metastatic MTC. The purpose of this study was to assess the safety, resulting immune responses and clinical activity of the DCs. DCs were injected into a groin lymph node at 3-week intervals. Monitoring included serial calcitonin tumor marker measurements, radiological imaging and immunological in vitro tests (T-cell interferon-gamma detection assay, T-cell cytotoxicity assay). Ten patients (median age 47 years, range 29-77) were enrolled. DC vaccinations were well-tolerated and safe. After a median follow-up of 11 months, (range 7-26), 3 (30%) of 10 patients had stable disease, while 7 (70%) of the patients progressed during treatment. In 2 patients with stable disease, calcitonin decreased below treatment levels, paralleled by a T-cell-mediated immune response. Notably, treatment with DCs pulsed with a combination of different tumor cell lysates was followed by a calcitonin decrease in 4 patients who had previously experienced a calcitonin increase during monotherapy with DCs pulsed with a single lysate. Allogeneic tumor cell lysate-based DC immunotherapy is well-tolerated and safe. Combined treatment with different tumor cell lysate-pulsed DCs increases the likelihood of a calcitonin tumor marker response and should therefore be preferred over monotherapy with DCs pulsed with a single lysate.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Aged -
- Calcitonin - blood
- Cancer Vaccines -
- Carcinoma, Medullary - diagnosis Carcinoma, Medullary - immunology Carcinoma, Medullary - secondary Carcinoma, Medullary - therapy
- Cell Line, Tumor -
- Dendritic Cells - immunology Dendritic Cells - transplantation
- Disease Progression -
- Female -
- Humans -
- Immunotherapy, Adoptive - adverse effects
- Interferon-gamma - metabolism
- Male -
- Middle Aged -
- Pilot Projects -
- T-Lymphocytes, Cytotoxic - immunology
- Thyroid Neoplasms - diagnosis Thyroid Neoplasms - immunology Thyroid Neoplasms - secondary Thyroid Neoplasms - therapy
- Time Factors -
- Transplantation, Autologous -
- Treatment Outcome -
- Tumor Markers, Biological - blood
- Find related publications in this database (Keywords)
- medullary thyroid carcinoma
- immunotherapy
- dendritic cells
- allogeneic tumor cell lysate