Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Silberhumer, GR; Zakian, K; Malhotra, S; Brader, P; Gönen, M; Koutcher, J; Fong, Y.
Relationship between 31P metabolites and oncolytic viral therapy sensitivity in human colorectal cancer xenografts.
Br J Surg. 2009; 96(7):809-816
Doi: 10.1002/bjs.6604
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Brader Peter
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- Abstract:
- Background: Studies using phosphorus magnetic resonance spectroscopy (AIRS) have pointed to the significance of phospholipid metabolite alterations as biochemical markers for tumour progression or therapy response. Methods: Spectroscopic imaging was performed in colorectal flank tumours in nude mice. In vivo tumour doubling times for each cell line were measured. In vivo sensitivity of each tumour line to treatment with and NV1020 oncolytic viruses was assessed. Correlations between vital sensitivity, and tumour doubling time and phosphorus MRS were estimated. Results: For G207 virus, in vitro cytotoxicity tests showed cell viability at multiplicities of infection (ratio of viral particles per tumour cell) of 0.1 on day 6 as follows: C85, less than 1 per cent; HCT8, I per cent; LS174T, 9 per cent; HT29, 18 per cent; and C18, 92 per cent. Respective values for NV1020 were 1, 18, 4, 18 and 86 per cent. The phosphoethanolamine to phosphocholine ratio was significantly lower in virus-sensitive than -insensitive cells, and was dependent on tumour doubling time. Conclusion: Alterations in membrane phospholipid metabolites that relate to proliferation of cancer cells affect the efficacy of oncolytic viral therapy. MRS proved a highly sensitive non-invasive tool for predicting the efficacy of viruses.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Cell Line, Tumor -
- Colorectal Neoplasms - metabolism Colorectal Neoplasms - therapy
- Escherichia coli - metabolism
- Genetic Vectors -
- Herpesvirus 1, Human - metabolism
- Magnetic Resonance Spectroscopy - methods
- Male -
- Mice -
- Mice, Nude -
- Neoplasm Transplantation -
- Oncolytic Virotherapy -
- Phospholipids - metabolism
- Phosphorus Isotopes - diagnostic use
- Radiopharmaceuticals - diagnostic use
- Sensitivity and Specificity -
- Transfection -
- Transplantation, Heterologous -