Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Andersen, GE; Gry, H; Kostner, GM.
A new micromethod for routine measurement of serum LDL-apolipoprotein B.
J Lipid Res. 1988; 29(3):377-380
Doi: 10.1016/S0022-2275(20)43544-8
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- Co-Autor*innen der Med Uni Graz
- Kostner Gerhard
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- Abstract:
- A new method for low density lipoprotein (LDL) (d 1.019-1.063 g/ml)-apolipoprotein B (apoB) determination has been developed, based on the fact that very low density and intermediate density lipoproteins (VLDL and IDL) contain apolipoprotein C-I (apoC-I), whereas this apolipoprotein is apparently absent in LDL. VLDL and IDL were quantitatively precipitated with a monospecific anti-apoC-I antibody whereafter LDL-apoB in the supernatant was quantitated by Laurell rocket electrophoresis. Over a wide range of cholesterol and triglyceride values there was a linear correlation with LDL-apoB values measured after ultracentrifugation. The method would be useful for routine measurements, especially in children, since only 25 microliter of serum is required, and for making the diagnosis of hyperapobetalipoproteinemia, which at present is complicated.
- Find related publications in this database (using NLM MeSH Indexing)
- Apolipoproteins B - blood
- Humans - blood
- Lipoproteins, LDL - blood