Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Hermann-Kleiter, N; Ghaffari-Tabrizi, N; Blumer, MJ; Schwarzer, C; Mazur, MA; Artner, I.
Lasp1 misexpression influences chondrocyte differentiation in the vertebral column.
Int J Dev Biol. 2009; 53(7): 983-991.
Doi: 10.1387/ijdb.072435nh
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Ghaffari Tabrizi-Wizsy Nassim
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- The mouse mutant wavy tail Tg(Col1a1-lacZ)304ng was created through transgene insertion and exhibits defects of the vertebral column. Homozygous mutant animals have compressed tail vertebrae and wedge-shaped intervertebral discs, resulting in a meandering tail. Delayed closure of lumbar neural arches and lack of processus spinosi have been observed; these defects become most prominent during the transition from cartilage to bone. The spina bifida was resistant to folic acid treatment, while retinoic acid administration caused severe skeletal defects in the mutant, but none in wild type control animals. The transgene integrated at chromosome 11 band D, in an area of high gene density. The insertion site was located between the transcription start sites of the Rpl23 and Lasp1 genes. LASP1 (an actin binding protein involved in cell migration and survival) was found to be produced in resting and hypertrophic chondrocytes in the vertebrae. In mutant vertebrae, temporal and spatial misexpression of Lasp1 was observed, indicating that alterations in Lasp1 transcription are most likely responsible for the observed phenotype. These data reveal a yet unappreciated role of Lasp1 in chondrocyte differentiation during cartilage to bone transition.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Cell Differentiation - genetics
- Chondrocytes - cytology
- Chondrogenesis - genetics
- Collagen - genetics
- Cytoskeletal Proteins -
- Female -
- Folic Acid - pharmacology
- Gene Expression -
- Homeodomain Proteins - genetics
- LIM Domain Proteins -
- Mice -
- Mice, Inbred C57BL -
- Mice, Mutant Strains -
- Mice, Transgenic -
- Mutagenesis, Insertional -
- Neoplasm Proteins - genetics
- Osteogenesis - genetics
- Paired Box Transcription Factors - genetics
- Phenotype -
- Pregnancy -
- Spine - abnormalities
- Tail - abnormalities
- Tretinoin - pharmacology
- Find related publications in this database (Keywords)
- transgene insertion
- cartilage bone transition
- folic acid
- retinoic acid
- collagen